A3221

Iodoacetamide

Single use vial of 56 mg

• Synonym(s): 2-Iodoacetamide, Monoiodoacetamide, alpha-Iodoacetamide
• Linear Formula: ICH₂CONH₂
• CAS Number: 144-48-9
• Molecular Weight: 184.96
• Beilstein: 1739080
• EC Number: 205-630-1
• MDL number: MFCD00008028
• PubChem Substance ID: 24890731
• NACRES: NA.56

Properties

• Quality Level: 200
• Assay: ≥99% (HPLC)
• form: powder
• packaging: vial of 56 mg (Single use)
• mp: 92-95 °C (lit.)
• solubility: H₂O: soluble 50 mg/mL, clear, colorless to faintly yellow
• storage temp.: 2-8°C
• SMILES string: NC(=O)CI
• InChI: 1S/C2H4INO/c3-1-2(4)5/h1H2,(H2,4,5)
• InChI key: PGLTVOMIXTUURA-UHFFFAOYSA-N

Description

Application

Iodoacetamide may be used as an alkylating reagent for cysteine residues in peptide sequencing and peptide/protein analytical analysis. It may be used in 2-D protein electrophoresis to reduce streaking and improve resolution by eliminating artifacts that could result from disulfide formation during electrophoresis. By virtue of its reaction with cysteine, iodoacetamide is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease. Iodoacetamide may be used in disulfide stress research.

Biochem/physiol Actions

Iodoacetamide acts as an alkylating reagent for cysteine residues in peptide sequencing. It is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibiting ribonuclease.

Packaging

Packaged in sealed ampules

Legal Information

Technology developed in partnership with Proteome Systems^™

Proteome Systems is a trademark of Proteome Systems Ltd

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H301 - H317 - H334 - H413
• Precautionary Statements: P261 - P264 - P273 - P280 - P301 + P310 - P302 + P352
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Chronic 4 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves