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A3660

Sigma-Aldrich

Apoferritin from equine spleen

CAS Number:
MDL number:
NACRES:
NA.25

Quality Level

form

glycerol solution (50% glycerol and 0.075 M NaCl)

packaging

vial of 50 mg

storage temp.

−20°C

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Application

Apoferritin is a gel filtration molecular weight marker that can be used in gel filtration chromatography and protein chromatography. Apoferritin has the ability to demetallate hemin and to metallate protoporphyrin IX.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Product A3660, Apoferritin from equine spleen?

    The approximate molecular weight of this product is 443,000.

  4. What is the concentration of Product A3660, Apoferritin from equine spleen?

    The concentration is roughly 25 mg/mL, according to the technical bulletin.

  5. Can Product A3660, Apoferritin from equine spleen, be sterilized?

    Yes. First the glycerol will need to be removed (typically by dialysis). Then a solution of apoferritin in 0.15M sodium chloride can be filtered through a 0.2 micron filter, as is done for Product No. A3641.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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    Ask a Scientist here.

Noëlle Carette et al.
Journal of inorganic biochemistry, 100(8), 1426-1435 (2006-06-20)
Earlier crystallographic and spectroscopic studies had shown that horse spleen apoferritin was capable of removing the metal ion from hemin (Fe(III)-protoporphyrin IX) [G. Précigoux, J. Yariv, B. Gallois, A. Dautant, C. Courseille, B. Langlois d'Estaintot, Acta Cryst. D50 (1994) 739-743;
David P Klebl et al.
Acta crystallographica. Section D, Structural biology, 76(Pt 4), 340-349 (2020-04-08)
Despite the great strides made in the field of single-particle cryogenic electron microscopy (cryo-EM) in microscope design, direct electron detectors and new processing suites, the area of sample preparation is still far from ideal. Traditionally, sample preparation involves blotting, which
Xindong Wang et al.
Biosensors & bioelectronics, 47, 171-177 (2013-04-11)
A novel sandwich electrochemical immunoassay was developed for ultrasensitive detection of avian leukosis virus subgroup J (ALVs-J) using graphene quantum dots (GQDs) and apoferritin-encapsulated Cu (Cu-apoferritin) nanoparticles for signal amplification. GQDs were used both for the conjugation of primary ALVs-J
David P Klebl et al.
Structure (London, England : 1993), 28(11), 1238-1248 (2020-08-20)
A host of new technologies are under development to improve the quality and reproducibility of cryoelectron microscopy (cryoEM) grid preparation. Here we have systematically investigated the preparation of three macromolecular complexes using three different vitrification devices (Vitrobot, chameleon, and a
Venkata P Dandey et al.
Journal of structural biology, 202(2), 161-169 (2018-01-26)
We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution

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