A3678

Sigma-Aldrich

Ammonium persulfate

for molecular biology, suitable for electrophoresis, ≥98%

Synonym(s):
Ammonium peroxydisulfate, PER, Ammonium peroxodisulfate, APS, AP
Linear Formula:
(NH4)2S2O8
CAS Number:
Molecular Weight:
228.20
EC Number:
MDL number:
eCl@ss:
38030421
PubChem Substance ID:
NACRES:
NB.22

Quality Level

grade

for molecular biology

vapor density

7.9 (vs air)

assay

≥98%

reaction suitability

reagent type: oxidant

application(s)

electrophoresis: suitable

anion traces

chloride (Cl-): <10 ppm

cation traces

Fe: <10 ppm
heavy metals (as Pb): <50 ppm

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

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General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.
Catalyst for acrylamide gel polymerization.

Packaging

25, 100 g in poly bottle

Analysis Note

Tested for use in acrylamide polymerization.

signalword

Danger

hazcat

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

storage_class_code

5.1B - Oxidizing hazardous materials

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Maurer HR
Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis (1978)
Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.
Fancy DA and Kodadek T
Proceedings of the National Academy of Sciences of the USA, 96, 6020-6020 (1999)
Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.
Liao IC et al.
Advances in Functional Materials, 23, 5833-5833 (2013)
Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions.
Chiang MY et al.
Biomaterials, 34, 9754-9754 (2013)
Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis.
Alvarez-Gonzalez CA, et al.
Fish Physiology and Biochemistry, 36, 29-29 (2010)
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