A4464

Enhanced Avian Reverse Transcriptase [eAMV^™ RT]

For reverse transcription at higher temperatures & rare mRNAs

• MDL number: MFCD01634820
• NACRES: NA.55

Properties

• Quality Level: 200
• form: crystalline powder
• usage: sufficient for 50 reactions
• feature: dNTPs included: no; hotstart: no
• concentration: 20 units/μL
• technique(s): RT-PCR: suitable
• color: colorless
• input: purified RNA
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

eAMV^™ Reverse Transcriptase is an enhanced form of Avian Myeloblastosis Virus (AMV) RT that synthesizes a DNA strand complementary to RNA, DNA, or an RNA:DNA hybrid. This exceptionally robust AMV RT has greater thermostability than standard AMV or Moloney murine leukemia virus(M-MLV) reverse transcriptase. eAMV^™ RT is an ideal enzyme for producing high-quality full-length cDNA from total RNA or poly(A)⁺ RNA and is also efficient at transcribing long targets.

Application

Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.

Enhanced Avian Reverse Transcriptase [eAMV^™ RT] has been used for reverse transcription of total RNA to synthesize cDNA during quantitative reverse transcription PCR (RT-qPCR) analysis.

Biochem/physiol Actions

Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.

Features and Benefits

• Greater sensitivity for low abundance mRNA
• Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
• Efficient generation of full-length cDNA, up to 14.1 kb
• Produces first strand cDNA ready for PCR amplification

Packaging

Provided with a vial of 10× reaction buffer.

Unit Definition

One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)_12-18 as a primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

eAMV is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves