Merck
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A4464

Sigma-Aldrich

Enhanced Avian Reverse Transcriptase [eAMV RT]

For reverse transcription at higher temperatures & rare mRNAs

MDL number:
NACRES:
NA.55

Quality Level

form

crystalline powder

usage

sufficient for 50 reactions

feature

dNTPs included: no
hotstart: no

concentration

20 units/μL

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

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General description

eAMV Reverse Transcriptase is an enhanced form of Avian Myeloblastosis Virus (AMV) RT that synthesizes a DNA strand complementary to RNA, DNA, or an RNA:DNA hybrid. This exceptionally robust AMV RT has greater thermostability than standard AMV or Moloney murine leukemia virus(M-MLV) reverse transcriptase. eAMV RT is an ideal enzyme for producing high-quality full-length cDNA from total RNA or poly(A)+ RNA and is also efficient at transcribing long targets.

Application

Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.
Enhanced Avian Reverse Transcriptase [eAMV RT] has been used for reverse transcription of total RNA to synthesize cDNA during quantitative reverse transcription PCR (RT-qPCR) analysis.

Biochem/physiol Actions

Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.

Features and Benefits

  • Greater sensitivity for low abundance mRNA
  • Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
  • Efficient generation of full-length cDNA, up to 14.1 kb
  • Produces first strand cDNA ready for PCR amplification

Packaging

Provided with a vial of 10× reaction buffer.

Unit Definition

One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Emily Schifano et al.
Virulence, 10(1), 1013-1025 (2019-11-28)
Calcium signaling can elicit different pathways involved in an extreme variety of biological processes. Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology. The Ca2+-ATPase
Y M Zhu et al.
British journal of cancer, 91(11), 1970-1976 (2004-11-17)
Interleukin-8/CXCL8 (IL-8) is a chemokine and angiogenic factor. Recently, IL-8 was identified as an autocrine growth factor in several human cancers. Here, we investigated the expression and function of IL-8 in lung cancer cells. The expressions of IL-8 and its
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 5-5 (1989)
Ciara Fox et al.
Cureus, 10(12), e3763-e3763 (2019-03-02)
Introduction Ischaemic preconditioning (IPC) is a phenomenon whereby tissues develop an increased tolerance to ischaemia and subsequent reperfusion if first subjected to sublethal periods of ischaemia. Despite extensive investigation of IPC, the molecular mechanism remains largely unknown. Our aim was
Emily Schifano et al.
Applied microbiology and biotechnology, 104(20), 8937-8948 (2020-09-03)
The probiotic bacteria are helpful for nutritional and therapeutic purposes, and they are commercially available in various forms, such as capsules or powders. Increasing pieces of evidence indicate that different growth conditions and variability in manufacturing processes can determine the

Related Content

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

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