A4941

Activin A human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, lyophilized powder, suitable for cell culture

• EC Number: 201-069-1
• MDL number: MFCD02685750
• NACRES: NA.32

Properties

• Quality Level: 200
• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: 1-10 ng/mL ED₅₀
• mol wt: dimer 26 kDa (containing 116 amino acid residues βA subunits)
• packaging: pkg of 10 μg; pkg of 5 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P08476
• storage temp.: −20°C
• Gene Information: human ... INHBA(3624)

Description

General description

Activin A gene with two exons is mapped to human chromosome 2q35. Activin A also known as inhibin α-subunit belongs to the TGF-β (transforming growth factor-β) superfamily of proteins and its expression is exclusive to gonads, placenta and adrenal cortex.

Application

Activin A human has been used as a constituent of modified barth′s saline used in in vitro induction of pronephros. It has also been used as a constituent of Roswell Park memorial institute (RPMI) media to induce pancreatic differentiation of multipotent dermal fibroblasts in vitro.

Biochem/physiol Actions

Activins play a vital role in the regulation of hormonogenesis, cellular homeostasis and differentiation programs. Elevated expression of activin A and B leads to the loss of muscle mass. Both activin A and B have a potential to act as a serum markers of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME).

Physical form

Lyophilized from 0.2 μm filtered solution in 10 mM sodium citrate, pH 3.0.

Analysis Note

The biological activity is determined by its ability to inhibit the proliferation of mouse MPC-11 cells.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable