Alkaline Phosphatase Blue Membrane Substrate Solution

sufficient for 400 mL working substrate


Quality Level




sufficient for 400 mL working substrate

storage temp.


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General description

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.


Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
  • to analyse direct alkaline phosphatase (AP) activity
  • to perform alkaline phosphatase assays with colorimetric system
  • to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC


12 - Non Combustible Liquids

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

The Short Isoform of NF-YA Belongs to the Embryonic Stem Cell Transcription Factor Circuitry
Dolfini D, et al.
Stem Cells, 30(11), 2450-2459 (2012)
María Del Carmen Ortuño-Costela et al.
Stem cell research, 36, 101418-101418 (2019-03-22)
A mouse iPSC line, IISHDOi005-A, generated from fibroblasts obtained from a mouse C57BL/6J with an age of 1 year and a half, has been obtained. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc were delivered using Sendai virus.
Generation of pig iPS cells: a model for cell therapy
Montserrat N, et al.
Journal of Cardiovascular Translational Research, 4(2), 121-130 (2011)
Nelson Pediatric Symptom-Based Diagnosis, 255-274 (2018)
Generation of a human iPSC line from a patient with Leigh syndrome
Galera T, et al.
Stem Cell Research, 16(1), 63-66 (2016)
Related Content
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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