B9285

5-Bromo-2′-deoxyuridine

BioUltra, ≥99%

• Synonym(s): 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
• Empirical Formula (Hill Notation): C₉H₁₁BrN₂O₅
• CAS Number: 59-14-3
• Molecular Weight: 307.10
• Beilstein: 30395
• EC Number: 200-415-9
• MDL number: MFCD00006529
• PubChem Substance ID: 24892132
• NACRES: NA.51

Properties

• product line: BioUltra
• Quality Level: 200
• Assay: ≥99%
• form: powder
• impurities: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• ign. residue: ≤0.1%
• mp: 191-194 °C (dec.) (lit.)
• solubility: NH₄OH: 0.1 M at 20 °C, clear, colorless
• cation traces: Al: ≤0.0005%; Ca: ≤0.01%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.05%; Pb: ≤0.001%; Zn: ≤0.0005%
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• Inchi Key: WOVKYSAHUYNSMH-RRKCRQDMSA-N

Description

General description

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light.

Application

5-Bromo-2′-deoxyuridine has been used:

• to label proliferating cells in pregnant mice in cell proliferation assay
• to study the time course of cell proliferation at various times following ischemia, to confirm re-entry of MKI67+ cells in cell cycle to germ cells
• in 5′-Bromo-2′Deoxyuridine (BrdUrd) staining of fibroblasts

Biochem/physiol Actions

5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H340 - H361fd
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 1B - Repr. 2
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves