Merck
All Photos(3)

C0406

Sigma-Aldrich

κ-Casein from bovine milk

≥70% (PAGE), lyophilized powder

Synonym(s):
Kappa-Casein
CAS Number:
EC Number:
MDL number:
NACRES:
NA.61

Quality Level

biological source

bovine milk

assay

≥70% (PAGE)

form

lyophilized powder

technique(s)

isoelectric focusing (IEF): suitable

storage temp.

−20°C

Gene Information

General description

Caseins constitute 80% of the total milk proteins in the cow. κ-Casein is one of the major allergens in cow milk. It is a glycosylated protein comprising galactose, galactosamine, and sialic acid. It exists as tri- or tetrasaccharides and occurs in different isoforms based on the number of oligosaccharides attached to it. κ-Casein has a hydrophobic N-terminus and a hydrophilic C-terminus region.

Application

κ-Casein from bovine milk has been used:
  • as a standard to quantify the casein concentration and to evaluate the ability of the bacterial strain to hydrolyze caseins released by HM Mozzarella
  • in LPS-depleted cow′s milk protein preparation for lymphoproliferation assay
  • as standard to quantify κ-casein concentration in skimmed milk samples by reversed-phase high-performance liquid chromatography (RP-HPLC)

Packaging

100, 250 mg in poly bottle
1 g in poly bottle

Biochem/physiol Actions

κ-Casein from bovine milk is an immunoglobulin E (IgE)-binding epitope belonging to the casein phosphoprotein family. κ-Casein is important to the electrostatic and steric stabilization of casein micelles suspensions. It resists calcium precipitation and therefore, stabilizes other caseins. κ-Casein has been a target of research for creating low-phenylalanine milk through gene modification, which is important for patients suffering from metabolic diseases such as phenylketonuria (PKU).

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Aprianita Aprianita et al.
The Journal of dairy research, 81(2), 183-192 (2014-03-07)
This experiment was conducted to determine the effects of diets supplemented with cottonseed oil, Acacia mearnsii-condensed tannin extract, and a combination of both on composition of bovine milk. Treatment diets included addition of cottonseed oil (800 g/d; CSO), condensed tannin from
Hongjun Yu et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(41), E9560-E9569 (2018-09-28)
The protein disaggregase ClpB hexamer is conserved across evolution and has two AAA+-type nucleotide-binding domains, NBD1 and NBD2, in each protomer. In M. tuberculosis (Mtb), ClpB facilitates asymmetric distribution of protein aggregates during cell division to help the pathogen survive
A study on bovine kappa-casein aggregation after the enzymatic action of chymosin.
Maria Eugenia Hidalgo., et al
Colloids Surf., 76, 556-563 (2010)
Caterina Villa et al.
Comprehensive reviews in food science and food safety, 17(1), 137-164 (2018-01-01)
Cow milk allergy is one of the most common food allergies in early childhood and often persists through adult life, forcing an individual to a complete elimination diet. Milk proteins are present in uncounted food products, such as cheese, yogurt
Presence of functional, autoreactive human milk-specific IgE in infants with cow's milk allergy.
Jarvinen KM., et al.
Clinical and Experimental Allergy (2011)

Related Content

HPLC Analysis of Caseins on BIOshell™ A400 Protein C4

-casein basis (electrophoresis), lyophilized powder; β-Casein from bovine milk, BioUltra, ≥98% (PAGE)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service