C3867

Collagen, Type I solution from rat tail

BioReagent, suitable for cell culture, sterile-filtered

• Synonym(s): Collagen from rat tail
• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• NACRES: NA.75

Properties

• biological source: rat tail
• Quality Level: 200
• description: Approx. 100mg/vial (Volume 22 - 25ml/vial)
• sterility: sterile-filtered
• product line: BioReagent
• Assay: >95% (SDS-PAGE)
• form: liquid
• mol wt: apparent mol wt 115-130 kDa by SDS-PAGE (doublet); apparent mol wt 215-235 kDa by SDS-PAGE (doublet)
• packaging: pkg of 1 vial
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• UniProt accession no.: P02454
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: rat ... Col1a1(29393)

Description

General description

Collagen is an extracellular matrix protein comprising triple helical structure. Homotrimetric collagens have three identical α-chains. Type I collagen have one α-chain different in sequence among the three. Type I collagen was first isolated from rat-tail tendon, has two main α chains (α and α ) and one β chain.

When C3867 is first removed from the cooler it will appear gelled. When it is allowed to come fully to ambient room temperature it will appear as a liquid.

Application

Collagen, Type I solution from rat tail has been used for coating culture plates and dishes for human hepatocyte HepG2 cell line and podocytes. It has also been used as a standard for total collagen content calibration curve generation for the quantification of liver extracellular matrix pre-gel.

Used as a coating material to support adherent cells growth and differentiation.

Biochem/physiol Actions

Collagen is essential for the mechanical integrity of tendons and bone. Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels. It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes.

Preparation Note

Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.

Sterile solution prepared from rat tail tendons by a modification of the published extraction method ¹. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method ².
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable