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C5670

Sigma-Aldrich

Calcium chloride

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

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Synonym(s):
CaCl2, Calcium dichloride
Linear Formula:
CaCl2
CAS Number:
Molecular Weight:
110.98
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.71

grade

anhydrous

Quality Level

vapor pressure

0.01 mmHg ( 20 °C)

product line

BioReagent

Assay

≥96.0%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | plant: suitable

mp

772 °C (lit.)

SMILES string

[Cl-].[Cl-].[Ca++]

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

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This Item
383147499609C4901
Calcium chloride anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sigma-Aldrich

C5670

Calcium chloride

Essential+ Grade
Calcium chloride desiccant, ACS reagent, ≥96.0%

Sigma-Aldrich

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Calcium chloride

Essential Grade
Calcium chloride anhydrous, powder, 99.99% trace metals basis

Sigma-Aldrich

499609

Calcium chloride

-
Calcium chloride anhydrous, powder, ≥97%

Sigma-Aldrich

C4901

Calcium chloride

-
vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

assay

≥96.0%

assay

≥96.0%

assay

99.99% trace metals basis

assay

≥97%

form

powder

form

powder

form

powder

form

powder

mp

772 °C (lit.)

mp

772 °C (lit.)

mp

772 °C (lit.)

mp

772 °C (lit.)

product line

BioReagent

product line

-

product line

-

product line

-

General description

Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10

Application

This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.

Preparation Note

This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

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