Merck
All Photos(1)

C5737

Sigma-Aldrich

Monoclonal Anti-Caspase 3 antibody produced in mouse

clone 84803.111, purified immunoglobulin, lyophilized powder

Synonym(s):
Anti-Apopain, Anti-Yama, Anti-CPP32
MDL number:

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

84803.111, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

western blot: 1.0 μg/mL using human Jurkat cells

isotype

IgG2b

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

CASP3 (caspase 3) is a cysteine protease belonging to the caspase protein family, and is related to interleukin-1β-converting enzyme (ICE). It is the human homologue of Caenorhabditis elegans Ced-3 protein. This protein forms a key element of apoptosis.

Specificity

Detects recombinant human precursor caspase 3 and the 18 kDa form produced during apoptosis.

Immunogen

recombinant 18 kDa subunit, amino acids 29-175, of human caspase 3.

Biochem/physiol Actions

CASP3 (caspase 3) is an essential protein of apoptosis, which includes multiple death-inducing signals, and caspase 3 functions at the last steps of programmed cell death. This protein is shown to be expressed in esophageal squamous cell carcinoma (ESCC), and its down-regulation is related to increased malignancy and reduced overall survival. Its expression in metastatic lymph nodes (LN) might serve as a marker for poor survival prognosis in patients with resected ESCC and metastasis to LN. Studies in multiple cancers such as, gastric, colorectal, ovarian and cervical cancers show that aberrant expression of this gene is linked with cancer progression and aggressive behavior. It might have potential as a marker to determine the prognosis of the above cancers.

Physical form

Lyophilized from a 0.2 μm filtered solution in 0.01 M phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Qian Hu et al.
International journal of clinical and experimental pathology, 7(8), 5057-5070 (2014-09-10)
Emerging evidence has indicated that apoptotic cells have a compensatory effect on the proliferation of neighboring cells. Recent studies have shown that apoptotic tumor cells stimulate the repopulation of tumors from a small number of surviving cells by cleaved caspase-3
Tingting Gu et al.
Oncology letters, 14(6), 6815-6822 (2018-02-10)
The present study explored the expression of tetraspanin 1 (TSPAN1) in esophageal carcinoma (EC) and its association with clinicopathological factors. TSPAN1 small interfering RNA (siRNA) was designed to target the TSPAN1 gene in Eca-109 cells in order to explore the
Xiu-Shen Wang et al.
World journal of gastroenterology, 20(15), 4414-4420 (2014-04-26)
To assess whether differential expression of caspase-3 in paired metastatic lymph nodes (LNs) is prognostic of survival in patients with resectable esophageal squamous cell carcinoma (ESCC). Capases-3 expression was evaluated immunohistochemically in 122 pairs of primary ESCCs and regional metastatic
Chuandong Zhu et al.
Oncology letters, 15(4), 4221-4228 (2018-03-20)
The high atomic number of gold nanoparticles (GNPs) enables them to offer potential as practical and efficient radiosensitizing agents for cancer radiotherapy applications. In the present study, it was demonstrated that GNPs can significantly modulate the irradiation response of hepatocellular
Lars Vereecke et al.
Nature communications, 5, 5103-5103 (2014-10-01)
The transcription factor NF-κB is indispensable for intestinal immune homeostasis, but contributes to chronic inflammation and inflammatory bowel disease (IBD). A20, an inhibitor of both NF-κB and apoptotic signalling, was identified as a susceptibility gene for multiple inflammatory diseases, including

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