C7642

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

FITC conjugate, Type IV, lyophilized powder

Synonym(s):
ConA
NACRES:
NA.32

Quality Level

type

Type IV

form

lyophilized powder

composition

Protein, 5-15% biuret

extent of labeling

3-6 mol FITC per mol protein

impurities

Carbohydrate, essentially free

conjugate

FITC conjugate

storage temp.

−20°C

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Packaging

2, 10 mg in glass bottle
Package size based on protein content

Application

For the fluorescent detection of glycoproteins containing α−D-mannose or α−D-glucose

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Physical form

Lyophilized powder containing phosphate buffer salts and NaCl

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

pictograms

Exclamation mark

signalword

Warning

hcodes

hazcat

Skin Sens. 1

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Li Liu et al.
Molecular biology and evolution, 37(8), 2300-2308 (2020-04-04)
Fisher's fundamental theorem of natural selection predicts no additive variance of fitness in a natural population. Consistently, studies in a variety of wild populations show virtually no narrow-sense heritability (h2) for traits important to fitness. However, counterexamples are occasionally reported...
Romain Kania et al.
Acta oto-laryngologica, 139(4), 345-350 (2019-02-27)
Biofilms are communities of bacteria embedded in a self-produced glycocalyx matrix. Adenoids have been shown to harbor bacterial biofilms. Aim/objectives: To compare the prevalence of biofilms in adenoid of children with chronic otitis media (COM) (group1) versus a control group...
Jianhong Zhou et al.
Biochemical pharmacology, 180, 114108-114108 (2020-06-23)
Noninfectious (autoimmune and immune-mediated) uveitis is an ocular inflammatory disease which can lead to blindness in severe cases. Due to the potential side effects of first-line drugs for clinical uveitis, novel drugs and targets against uveitis are still urgently needed....
Hyeong Rae Kim et al.
Biosensors & bioelectronics, 170, 112656-112656 (2020-10-04)
Point-of-care risk assessment (PCRA) for airborne viruses requires a system that can enrich low-concentration airborne viruses dispersed in field environments into a small volume of liquid. In this study, airborne virus particles were collected to a degree above the limit...
Sanda Ljubicic et al.
Biology of the cell, 112(12), 427-438 (2020-08-29)
Cell-cell or cell-substrate interactions are lost when cells are dissociated in culture, or during pathophysiological breakdowns, therefore impairing their structure and polarity, and affecting their function. We show that single rat β-cells, cultured under non-adhesive conditions, form intracytoplasmic vacuoles increasing...

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