Merck
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C7752

Sigma-Aldrich

Cytochrome c from equine heart

≥95% based on Mol. Wt. 12,384 basis

Synonym(s):
Cytochrome c from horse heart
CAS Number:
EC Number:
MDL number:
NACRES:
NA.61

Quality Level

biological source

horse heart

assay

≥95% (SDS-PAGE)
≥95% based on Mol. Wt. 12,384 basis

form

powder

mol wt

12,384

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

bovine ... CSN1S2(282209)

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Application

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome c.
The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.

Packaging

50, 100, 500 mg in glass bottle
1, 5 g in glass bottle

Biochem/physiol Actions

Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen

Preparation Note

Prepared with acetic acid without using TCA.

Other Notes

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. I see that you list several horse heart cytochrome c products. What are the differences, and which one should I choose?

    Product No. C2506 is prepared with trichloroacetic acid (TCA), whereas C7752 is prepared with the milder acetic acid. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Product No. C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. It is recommended when an ultra-high purity is required.  Product No. C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. This fraction shows a high rate of ascorbic acid oxidation, but low activity in a cytochrome oxidase system.  Product No. C7150 is also prepared from C7752; it is suitable for use as a gel filtration molecular weight marker.

  6. How do I determine how much of Product C7752, Cytochrome c, is in the oxidized form, and how much is reduced?

    This is determined by a spectral assay. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service (techserv@sial.com).

  7. Is Product C7752, Cytochrome c, predominantly in the oxidized form or the reduced form?

    This product is predominantly (>95%) in the oxidized form.

  8. How should I store solutions of Product C7752, Cytochrome c?

    Our general recommendations are as follows:  Store at -20 °C (freezer) for up to six months. Store at 2-8 °C (refrigerator) for up to two weeks. Store at 20-25 °C (room temperature) for up to three days. According to J. Biol. Chem, 237, 2161-2174 (1962), cytochrome c is stable to boiling, and over an unusually wide range of pH values. However, it denatures readily at extreme pH (low or high), and in high concentrations of organic solvents, such as ethanol or acetone.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Marilena Carbone et al.
Data in brief, 28, 104842-104842 (2019-12-24)
We present SEM, ThT fluorescence and circular dichroism (CD) data of amyloidogenic aggregates of cytochrome c (cyt c).This protein is of outmost relevance in many biochemical processes, such as respiratory chain in mitochondria and cells apoptosis. The present data focus
Dmitry Malyshka et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 23(5), 1151-1156 (2016-11-20)
Ferricytochrome c binding to cardiolipin-containing liposomes produces a heterogeneous distribution of conformations comprising native-like and non-native misfolded proteins. We utilized the photoreduction of native ferricytochrome c in the presence of potassium ferrocyanide and resonance Raman spectroscopy to probe the population of native
Lara Massai et al.
Journal of inorganic biochemistry, 165, 1-6 (2016-10-11)
Three Pd(II) and Pt(II) complexes with chelating mono(imidazolin-2-imine) and bis(imidazolin-2-imine) ligands i.e. [Pd(DMEAImiPr)Cl2] (1) (DMEAImiPr, 2-(1,3-diisopropyl-4,5-dimethylimidazolin-2-imine)ethan-1-dimethylamine), [Pd(DACH(ImiPr)2)Cl2] (2) (DACH(ImiPr)2, N,N'-(cyclohexane-1,2-diyl)bis(1,3-diisopropyl-4,5-dimethylimidazolin-2-imine)) and [Pt(DMEAImiPr)Cl2] (3), are evaluated here as potential cytotoxic and anticancer agents. An acceptable solution behaviour was found for the
Valentina Peleh et al.
BMC biology, 15(1), 106-106 (2017-11-10)
Many proteins of the mitochondrial intermembrane space (IMS) contain structural disulfide bonds formed by the mitochondrial disulfide relay. In fungi and animals, the sulfhydryl oxidase Erv1 'generates' disulfide bonds that are passed on to the oxidoreductase Mia40, which oxidizes substrate
A H Mulder et al.
Clinical and experimental immunology, 98(2), 270-278 (1994-11-01)
ANCA have been demonstrated to induce the respiratory burst in primed neutrophils. In this study we have extended the investigations on neutrophil activation by ANCA directed against proteinase 3 (PR3), myeloperoxidase (MPO) and lactoferrin (LF), and we have analysed the

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