Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare. In addition, C9210 is a cross-linked resin and C9142 is not.
C9210
Cyanogen bromide-activated Agarose
lyophilized powder
Synonym(s):
Agarose, cyclic carbonimidate, CNBr-Activated Agarose
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form
lyophilized powder
Quality Level
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix active group
cyanate or related structures
matrix spacer
1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling
1 g swells to 2.5-4.5 mL
capacity
≥10 mg/mL binding capacity (BSA)(via amino groups)
suitability
suitable for chromatography
storage temp.
−20°C
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This Item | SRE0039 | C7013 | A6112 |
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) - | technique(s) affinity chromatography: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form saline suspension |
| suitability suitable for chromatography | suitability suitable for chromatography | suitability - | suitability suitable for chromatography |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| matrix cross-linked 4% beaded agarose | matrix cross-linked 4% beaded agarose | matrix cross-linked 4% beaded agarose | matrix 4% beaded agarose |
Application
Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.
Physical form
Lyophilized powder stabilized with lactose
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support.
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
Current Protocols in Molecular Biology, 94, 21-21 (2011)
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
Immobilization of solubilized UDP-glucuronosyltransferase from rat liver microsomes to Sepharose 4B.
R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of
Related Content
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What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?
1 answer-
The control for this product is Product No. CL4B200 (Sepharose CL-4B).
Helpful?
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When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?
1 answer-
The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.
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