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C9409

Sigma-Aldrich

Creatinine Deiminase microbial

lyophilized powder, ≥25 units/mg protein

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Synonym(s):
Creatinine Deaminase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥25 units/mg protein

mol wt

~260 kDa

composition

Protein, ≥15% biuret

storage temp.

−20°C

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This Item
C3172C3921C2409
specific activity

≥25 units/mg protein

specific activity

100-300 units/mg protein

specific activity

10-15 units/mg protein

specific activity

≥4 units/mg solid

mol wt

~260 kDa

mol wt

~175 kDa

mol wt

45 kDa

mol wt

~100 kDa

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

composition

Protein, ≥15% biuret

composition

Protein, 65-85%

composition

Protein, ~70% biuret

composition

-

Application

Creatinine Deiminase microbial has been used:
  • to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
  • in creating creatinine-sensing membrane for biophysical studies
  • to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples

Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

Biochem/physiol Actions

Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.

Physical properties

Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)

Unit Definition

One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.

Physical form

Lyophilized powder containing mannitol as stabilizer

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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C M Huang et al.
Clinical chemistry, 34(1), 59-62 (1988-01-01)
We developed an enzymatic method for determination of 5-fluorocytosine in serum, using creatine iminohydrolase (EC 3.5.4.21), the Cobas-Bio analyzer, and an extant ammonia method. Analytical recovery (y) of drug added to serum (x) was good, with y = 0.97x-0.7, Sy.x
Anne K Bendt et al.
Archives of microbiology, 181(6), 443-450 (2004-05-19)
In order to utilize different nitrogen sources and to survive situations of nitrogen limitation, microorganisms have developed several mechanisms to adapt their metabolism to changes in the nitrogen supply. In this communication, the use of creatinine as an alternative nitrogen
R G Washburn et al.
The Journal of antimicrobial chemotherapy, 17(5), 673-677 (1986-05-01)
Creatinine iminohydrolase (EC 3.5.4.21) quantitatively releases ammonia from flucytosine (5FC) as well as from creatinine. Using 39 sera from eight patients receiving the combination of amphotericin B with 5FC, we demonstrated that this rapid enzymatic reaction provides a valid measure
Hong Dinh Duong et al.
Sensors (Basel, Switzerland), 17(11) (2017-11-09)
In this study, the oxazine 170 perchlorate (O17)-ethylcellulose (EC) membrane was successfully exploited for the fabrication of creatine- and creatinine-sensing membranes. The sensing membrane exhibited a double layer of O17-EC membrane and a layer of enzyme(s) entrapped in the EC
Manuel Gutiérrez et al.
Biosensors & bioelectronics, 23(6), 795-802 (2007-10-13)
Urea and creatinine biosensors based on urease and creatinine deiminase, respectively, covalently immobilized onto ammonium selective electrodes, were included in an array together with sensors sensitive to ammonium, potassium and sodium. Generic sensors to alkaline ions were also included. All

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