All Photos(3)

D4184

Sigma-Aldrich

JumpStart Taq DNA Polymerase

without MgCl2

MDL number:
NACRES:
NA.55

Quality Level

form

liquid

usage

sufficient for 1500 reactions
sufficient for 250 reactions
sufficient for 50 reactions

feature

dNTPs included: no
hotstart

concentration

2.5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

The Taq DNA polymerase activity is inactivated by combining the enzyme with JumpStart Taq antibody, a neutralizing monoclonal antibody to Taq DNA polymerase. Antibody inactivation provides a simple, efficient procedure for hot-start polymerase chain reaction (PCR). Hot start PCR can significantly improve the results of DNA amplification by reducing the generation of nonspecific amplification products and primer-dimer artifacts. When used in PCR, JumpStart Taq DNA polymerase is inactive at low (room) temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active.

Application

JumpStart Taq DNA polymerase has been used:
  • In the PCR amplification of DNA isolated from herbarium specimens.
  • In the PCR reaction mixture for randomly amplified polymorphic DNA polymerase chain reaction (RAPD PCR).
  • To amplify and detect a point mutation in the EGFR exon 19 using specific cancer cell lines.
  • In real-time quantitative PCR
  • For PCR amplification of complex genomic or cDNA templates
  • For the PCR amplification of very low-copy number targets
  • For the PCR amplification of many thermal cycles (>35), and multiple primer pairs in the same reaction tube
  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers

Features and Benefits

  • Reduces non-specific amplification
  • Increases PCR specificity and yield
  • Reduces set-up time concerns associated with manual or wax Hot Start methods
  • Activation time of less than 1 minute

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.
Supplied with 10× reaction buffer without MgCl2. Includes a separate tube of 25 mM MgCl2

Other Notes

Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

James L Lordan et al.
Journal of immunology (Baltimore, Md. : 1950), 169(1), 407-414 (2002-06-22)
In sensitized individuals, exposure to allergens such as Dermatophagoides pteronyssinus (Der p) causes Th2 polarization and release of cytokines, including IL-4 and IL-13. Because Der p extracts also have direct effects on epithelial cells, we hypothesized that allergen augments the
I A Afonina et al.
BioTechniques, 32(4), 940-944 (2002-04-19)
Here we describe the properties of a novel class of oligonucleotide probes capable of sensitive hybridization-triggered fluorescence. These fluorogenic probes, known commercially as MGB Eclipse probes, are characterized by having a conjugated minor groove binder (MGB) ligand at the 5'-end
Julian R Starr et al.
Molecular ecology resources, 9 Suppl s1, 151-163 (2009-05-01)
We investigate the species discriminatory power of a subset of the proposed plant barcoding loci (matK, rbcL, rpoC1, rpoB, trnH-psbA) in Carex, a cosmopolitan genus that represents one of the three largest plant genera on earth (c. 2000 species). To
Julia K Veir et al.
Veterinary therapeutics : research in applied veterinary medicine, 8(4), 229-238 (2008-01-10)
T evaluate the effect of supplementation with Enterococcus faecium strain SF68 (NCIMB10415) on immune function, responses to a multivalent vaccine were investigated in kittens given palatability enhancer with or without E. faecium SF68 daily. E. faecium SF68 was detected in
Isabelle Desitter et al.
Anticancer research, 31(2), 427-441 (2011-03-08)
Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells. The ScreenCell® devices are single-use and low-cost innovative devices that use a filter

Articles

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Protocols

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

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End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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