D4902

Dexamethasone

powder, BioReagent, suitable for cell culture, ≥97%

• Synonym(s): (11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione, 9α-Fluoro-16α-methyl-11β,17α,21-trihydroxy-1,4-pregnadiene-3,20-dione, 9α-Fluoro-16α-methylprednisolone, Prednisolone F
• Empirical Formula (Hill Notation): C₂₂H₂₉FO₅
• CAS Number: 50-02-2
• Molecular Weight: 392.46
• Beilstein: 2066651
• EC Number: 200-003-9
• MDL number: MFCD00064136
• PubChem Substance ID: 24893906
• NACRES: NA.75

Properties

• biological source: synthetic
• Quality Level: 200
• product line: BioReagent
• assay: ≥97%
• form: powder
• potency: 4-500 ng/mL
• technique(s): cell culture | mammalian: suitable
• mp: 262-264 °C (lit.)
• solubility: methanol: 25 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@]4(C)[C@@]3(F)[C@@H](O)C[C@]2(C)[C@@]1(O)C(=O)CO
• InChI: 1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,24,26,28H,4-5,8,10-11H2,1-3H3/t12-,15+,16+,17+,19+,20+,21+,22+/m1/s1
• InChI key: UREBDLICKHMUKA-CXSFZGCWSA-N
• Gene Information: human ... ABCB1(5243) , CYP3A4(1576) , IL4(3565) , IL5(3567) , NR3C1(2908) ; mouse ... Abcb1a(18671) , Abcb1b(18669) , Ifng(15978) , Nos2(18126) , Ptgs2(19225) , Tnf(21926) ; rat ... Ar(24208) , Nr3c1(24413) , Tnf(24835)

Description

Application

Dexamethasone is used to study apoptosis, cell signaling pathways, cell differentiation and gene expression.

Packaging

25, 100, 500 mg in glass bottle

1 g in glass bottle

Bottomless glass bottle. Contents are inside inserted fused cone.

Biochem/physiol Actions

Glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth, and differentiation. Inhibits the induction of nitric oxide synthase.

It is poorly metabolized in the body and has great affinity and bioavailability.

Physical form

powder 2-8 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 20 μg/ml stock solution, add 1ml absolute ethanol per mg product; gently swirl to dissolve. Add 49 ml sterile medium per ml of ethanol added, while mixing, to acheive final concentration of 20 μg/ml.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H360FD
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves