Merck
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D7656

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from salmon testes

For hybridization

CAS Number:
MDL number:
eCl@ss:
32160414
NACRES:
NA.52

Quality Level

grade

for molecular biology

form

solution

concentration

10.0 ± 1.0 mg/mL in H2O

shipped in

dry ice

storage temp.

−20°C

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General description

Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon.

Application

Deoxyribonucleic acid, single stranded from salmon testes has been used for/as:
  • in situ hybridization histochemistry of rat brain, human kidney sections and renal biopsy tissues
  • preparing standard curve of DNA
  • prehybridization solution in southern and northern hybridizations
  • dilution of the cDNA
  • fluorescent in-situ hybridization (FISH)
  • ChIP analysis
  • a standard to analyse the degraded nucleosides of genomic DNA by high performance liquid chromatography
  • a component of hybridization solution in FISH
  • a control in DNA-DNA hybridization experiment between various bacterial species
  • to precipitate probes in fluorescence in situ hybridization (FISH)
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Suitable for use as a blocking agent in Southern hybridizations.

Preparation Note

This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Other Notes

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Similar rates of chromosomal aberrant secondary oocytes in two indigenous cattle (Bos taurus) breeds as determined by dual-color FISH
Pauciullo, Alfredo, et al
Theriogenology, 675-683 (2012)
Frequency of aneuploidy in in vitro-matured MII oocytes and corresponding first polar bodies in two dairy cattle (Bos taurus) breeds as determined by dual-color fluorescent in situ hybridization
Nicodemo, D., et al
Theriogenology, 523-529 (2010)
Sydney Tang et al.
The Journal of clinical investigation, 111(4), 515-527 (2003-02-18)
Renal tubulointerstitial injury is characterized by inflammatory cell infiltrate; however, the stimuli for leukocyte recruitment are not fully understood. IL-8 is a potent chemokine produced by proximal tubular epithelial cells (PTECs). Whether nephrotic proteins stimulate tubular IL-8 expression remains unknown.
XY aneuploidy rate in sperm of two ?minor? breeds of cattle (Bos taurus) by using dual color fluorescent in situ hybridization (FISH)
Pauciullo, Alfredo, et al
Theriogenology, 688-695 (2010)
L Kölby et al.
British journal of cancer, 89(7), 1383-1388 (2003-10-02)
The radio-iodinated noradrenaline analogue meta-iodobenzylguanidine (MIBG) can be used for scintigraphy and radiation therapy of neuroendocrine (NE). The aim of the present study was to study the importance of vesicular monoamine transporters (VMATs) for the uptake of (123)I-MIBG in NE

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