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D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

Synonym(s):

Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO

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About This Item

Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

product line

BioReagent

Assay

≥99.9%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

technique(s)

DNA sequencing: suitable
PCR: suitable
transfection: suitable

impurities

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

absorption

passes test

suitability

suitable for molecular biology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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General description

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
Claudia C Faria et al.
Cancer research, 75(1), 134-146 (2014-11-14)
Medulloblastoma is the most common malignant pediatric brain tumor, with metastases present at diagnosis conferring a poor prognosis. Mechanisms of dissemination are poorly understood and metastatic lesions are genetically divergent from the matched primary tumor. Effective and less toxic therapies
Mike Clements et al.
Toxicological sciences : an official journal of the Society of Toxicology, 140(2), 445-461 (2014-05-09)
Human stem cell derived cardiomyocytes (hESC-CM) provide a potential model for development of improved assays for pre-clinical predictive drug safety screening. We have used multi-electrode array (MEA) analysis of hESC-CM to generate multi-parameter data to profile drug impact on cardiomyocyte
Mulmudi Hemant Kumar et al.
Advanced materials (Deerfield Beach, Fla.), 26(41), 7122-7127 (2014-09-13)
Lead free perovskite solar cells based on a CsSnI3 light absorber with a spectral response from 950 nm is demonstrated. The high photocurrents noted in the system are a consequence of SnF2 addition which reduces defect concentrations and hence the

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