The specific salmon species is not determined for this product. See the link below to review a sample Certificate of Origin for this material:
https://www.sigmaaldrich.com/coo/undefined/D9156/SLBM7249V.
D9156
Deoxyribonucleic acid, single stranded from salmon testes
For hybridization
Synonym(s):
single-stranded template DNA
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About This Item
Assay:
9-11 mg/mL (DNA concentration)
Biological source:
fish testis (salmon)
Form:
solution
Storage temp.:
−20°C
Pricing and availability is not currently available.
biological source
fish testis (salmon)
Quality Level
grade
Molecular Biology
assay
9-11 mg/mL (DNA concentration)
form
solution
concentration
10 mg/mL in H2O
shipped in
dry ice
storage temp.
−20°C
General description
A ready-to-use solution of high quality single-stranded template DNA isolated from the testes of salmon. The solution is supplied at a concentration of 9 - 12 mg/ml .
Application
Deoxyribonucleic acid, single stranded from salmon testes is suitable for use as a blocking agent in Southern hybridizations. It was used for in situ hybridization histochemistry of bone marrow biopsy samples[1] and human blood monocyte subpopulations.[2]
For use as a blocking agent in Northern and Southern blotting.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Preparation Note
This DNA is ethanol precipitated and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
1 of 1
This Item | |||
|---|---|---|---|
| biological source fish testis (salmon) | biological source fish testis | biological source fish | biological source bovine thymus |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology, puriss. p.a. | grade Molecular Biology |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
| assay 9-11 mg/mL (DNA concentration) | assay 9-11 mg/mL | assay - | assay 9-11 mg/mL (DNA concentration) |
| form solution | form solution | form lyophilized powder | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
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Instructions
H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
We have recently demonstrated the presence of Kaposi's sarcoma-associated herpesvirus (KSHV) in cultured bone marrow (BM) stromal dendritic cells from all patients with myeloma studied. To show that these findings were not an artifact of tissue culture, we performed in
Marion Herbette et al.
PLoS genetics, 17(7), e1009662-e1009662 (2021-07-07)
Segregation Distorter (SD) is a male meiotic drive system in Drosophila melanogaster. Males heterozygous for a selfish SD chromosome rarely transmit the homologous SD+ chromosome. It is well established that distortion results from an interaction between Sd, the primary distorting
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D9156-5ML | 04061833590362 |
| D9156-1ML | 04061833590355 |
| D9156-5X1ML | 04061833590379 |
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What Salmon sp. D9156 is prepared from?
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