D9307

Sigma-Aldrich

JumpStart Taq DNA Polymerase

with MgCl2

MDL number:

Quality Level

form

liquid

feature

dNTPs included: no
hotstart

concentration

2.5 units/μL

application(s)

PCR: suitable

color

colorless

Featured Industry

Agriculture

shipped in

wet ice

storage temp.

−20°C

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Application

JumpStart Taq DNA Polymerase has been used in PCR (polymerase chain reaction) and to amplify DNA extracted from formaldehyde fixed paraffin embedded (FFPE) samples.
  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers

Features and Benefits

  • Reduces non-specific amplification
  • Increases PCR specificity and yield
  • Reduces set-up time concerns associated with manual or wax Hot Start methods
  • Activation time of less than 1 minute

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.
Supplied with 10× reaction buffer containing 15 mM MgCl2

Other Notes

Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 2

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

High quality bisulfite sequencing using nanogram amounts of genomic DNA
Sun J, et al.
International journal of biochemistry and biotechnology, 2, 449-456 (2013)
Seohee Chang et al.
Biomolecules, 10(4) (2020-04-03)
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Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 793- 798 (2019)
Germán Gastón Leparc et al.
Nucleic acids research, 35(21), e146-e146 (2007-11-15)
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