All Photos(5)

DNB100

Sigma-Aldrich

GenElute Soil DNA Isolation Kit

greener alternative
NACRES:
NA.52

greener alternative product characteristics

Designing Safer Chemicals
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned

shipped in

ambient

storage temp.

room temp

General description

Sigma′s GenElute Soil DNA Isolation Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

  • Isolate total DNA from all microorganisms found in soil, including bacteria, fungi and algae
  • Process all types of soil, including common soil samples and difficult samples with high humic acid
  • Remove all humic acids using the provided Humic Acid Removal Columns and the OSR Solution
  • Isolated DNA is free of inhibitors such as humic acid - ready for PCR applications
  • Easy- to-use beads for extraction - fast without any time consuming sample grinding

Application

  • Quantitative, real-time PCR
  • Conventional PCR
  • Southern Blot Analysis
GenElute Soil DNA Isolation Kit has been used to extract microbial DNA from anodic biofilms of microbial fuel cells.

Features and Benefits

  • Process all types of soil - All types of soil samples can be processed with this kit, including common soil samples such as clay, loam and sandy soil as well as difficult soil samples with high humic acid content such as compost and manure.
  • Remove all humic acid from DNA samples - The kit removes all traces of humic acid using the provided Humic Acid Removal Columns and the OSR Solution
  • Rapid detection of microorganisms in soil samples - Isolate total DNA from all microorganisms found in soil, including bacteria, fungi and algae.
  • Fast and easy processing - Rapid spin column format allows for the isolation of DNA in under 30 minutes.
  • Isolate high quality total DNA - The purified DNA is free from all inhibitors including humic acid, and ca be used directly in downstream applications including PCR.

Unit Definition

Preps

Other Notes

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

Storage Class Code

8A - Combustible, corrosive hazardous materials

Certificate of Analysis

Certificate of Origin

Bin Gong et al.
Scientific reports, 9(1), 9377-9377 (2019-06-30)
Mangrove swamp is one of the world's richest and most productive marine ecosystems. This ecosystem also has a great ecological importance, but is highly susceptible to anthropogenic disturbances. The balance of mangrove ecosystem depends largely on the microbial communities in...
Enhanced electricity generation performance and dye wastewater degradation of microbial fuel cell by using a petaline NiO@ polyaniline-carbon felt anode
Dengjie Zhong
BioResources, 258 (2018)
Yazhou Lu et al.
Archives of microbiology, 202(7), 1717-1728 (2020-04-22)
Paeonia ludlowii is indigenous to Tibet and has an important ecological and economic value in China. In Tibet, P. ludlowii has been used in folk medicine with relative success. Plant microbial endophytes play an important role in plant growth, health...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service