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FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

FGF2, bFGF, Fibroblast Growth Factor-Basic human
CAS Number:
MDL number:

Quality Level

biological source



expressed in E. coli


≥97% (SDS-PAGE)


lyophilized powder


0.05-0.6 ng/mL

mol wt

16.0 kDa


pkg of 4X25 μg
pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles


cell culture | mammalian: suitable


≤1.00 EU/μg


white to faint yellow cast


water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt accession no.

storage temp.


Gene Information

human ... FGF2 (2257)

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General description

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.


Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).

Physical form

Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.

Analysis Note

The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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