F9037

Formamide

≥99.5% (GC), BioReagent, for molecular biology

• Synonym(s): Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• EC Number: 200-842-0
• MDL number: MFCD00007941
• PubChem Substance ID: 24894985

Properties

• Quality Level: 200
• grade: for molecular biology
• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• product line: BioReagent
• assay: ≥99.5% (GC)
• form: liquid
• autoignition temp.: 932 °F
• technique(s): electrophoresis: suitable; immunohistochemistry: suitable
• refractive index: n20/D 1.447 (lit.)
• pH: 4-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• density: 1.134 g/mL at 25 °C (lit.)
• suitability: suitable for nucleic acid hybridization
• storage temp.: 2-8°C
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

General description

Formamide is a clear liquid amide derived from formic acid. Formamide allows for the denaturation and renaturation of nucleic acids at room temperature.

Application

Formamide is suitable for use in hybridization buffers made using standard recipes. Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other applications include:

• Preparation of brain sections of mice for BrdU immunohistochemistry
• FISH analysis of HeLa cells.
• Preparation of the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis(The Merck Index, 12th ed).

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Features and Benefits

• Deionized for molecular biology applications
• Packaged under argon gas in amber bottles to protect from oxidation
• Used for DNA renaturation or DNA-RNA hybridization
• Reduces thermal stability of double stranded nucleic acids

Suitability

• nucleic acid hybridization
• cryoprotectant and gel-stabilizer
• destabilize nucleic acid duplexes
• elimination of secondary structure of nucleic acids
• preparation of hybridization buffers for RNA and DNA application

Other Notes

The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H351 - H360D - H373
• Precautionary Statements: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Target Organs: Blood
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): 305.6 °F
• Flash Point(C): 152 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves