G2150

L-Glutamine solution

200 mM, Hybri-Max^™, sterile-filtered, suitable for hybridoma

• Synonym(s): L-Glutamine for cell culture
• CAS Number: 56-85-9
• MDL number: MFCD00008044
• eCl@ss: 32160406
• PubChem Substance ID: 24895088
• NACRES: NA.75

Properties

• grade: Hybri-Max^™
• sterility: sterile-filtered
• Assay: ≥99% (TLC)
• form: solution
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• color: clear colorless
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: N[C@@H](CCC(N)=O)C(O)=O
• InChI: 1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
• InChI key: ZDXPYRJPNDTMRX-VKHMYHEASA-N
• Gene Information: rat ... Ggt1(116568)

Description

General description

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Application

The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4⁺ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.

Preparation Note

Prepared in cell culture grade water.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable