Merck
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G6511

Sigma-Aldrich

Glutathione S-Transferase from equine liver

lyophilized powder, ≥25 units/mg protein

All Photos(1)
Synonym(s):
GST, Glutathione R-transferase, Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase
CAS Number:
50812-37-8
Enzyme Commission number:
2.5.1.18 (BRENDA, IUBMB)
MDL number:
MFCD00131198
NACRES:
NA.47

biological source

equine liver

form

lyophilized powder

specific activity

≥25 units/mg protein

mol wt

45-50 kDa

composition

Protein, ≥60%

storage temp.

−20°C

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Glutathione S-Transferase from equine liver

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G3664

Glutathione Reductase from baker's yeast (S. cerevisiae)

biological source

equine liver

biological source

Porcine heart

biological source

bovine liver

biological source

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

mol wt

45-50 kDa

mol wt

-

mol wt

-

mol wt

118 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

specific activity

≥25 units/mg protein

specific activity

≥75 units/mg protein

specific activity

≥20 units/mg protein

specific activity

100-300 units/mg protein (biuret)

General description

Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. These isozymes differ in their catalytic activity, as well as in their non-catalytic binding properties. Cytoplasmic isoforms of GST are encoded by five genes, namely α, θ, μ, σ and π. α, μ and π are the most abundant forms in mammals. Membrane bound GST forms are encoded by a single gene.

Biochem/physiol Actions

Glutathione S-transferase (GST) from equine liver has been used-
  • as a constituent of Tris buffer for incubation of human umbilical vein endothelial cells (HUVEC) with atracurium to assess the proliferation of HUVEC in the presence of atracurium
  • as a component of GSB stock solution to determine GSB (glutathione S-bimane) conjugate fluorescence intensity in intact Arabidopsis cells
  • as an enzyme standard in spectrophotometric assay to determine the activity of GST
Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.

Unit Definition

One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.

Physical form

Lyophilized powder containing Tris, reduced glutathione and EDTA.

Analysis Note

Protein determined by biuret.
Purified and assayed by a modification of the method of Simons and Vander Jagt.
Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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