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G7126

Sigma-Aldrich

Glycine

ReagentPlus®, ≥99% (HPLC)

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Synonym(s):
Aminoacetic acid, Aminoethanoic acid, Glycocoll
Linear Formula:
NH2CH2COOH
CAS Number:
Molecular Weight:
75.07
Beilstein:
635782
EC Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26

product line

ReagentPlus®

Assay

≥99% (HPLC)

form

powder

technique(s)

western blot: suitable

impurities

≤0.01% Ammonia

loss

≤0.2% loss on drying

color

white to off-white

pKa (25 °C)

(1) 2.35, (2) 9.60
2.35

mp

240 °C (dec.) (lit.)

solubility

H2O: 200 mg/mL, clear, colorless to faintly yellow

cation traces

≤0.01% (Ammonia (NH3))

functional group

amine
carboxyl

storage temp.

room temp

SMILES string

NCC(O)=O

InChI

1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)

InChI key

DHMQDGOQFOQNFH-UHFFFAOYSA-N

Gene Information

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1 of 4

This Item
G7403V90014415527
vibrant-m

G7126

Glycine

-
vibrant-m

G7403

Glycine

Premium Grade
vibrant-m

V900144

Glycine

Essential Grade
vibrant-m

15527

Glycine

Essential Grade
form

powder

form

powder

form

powder

form

crystalline powder

assay

≥99% (HPLC)

assay

≥99% (titration)

assay

98%

assay

99-101% (based on anhydrous substance)

technique(s)

western blot: suitable

technique(s)

electron microscopy: suitable

technique(s)

cell culture | plant: suitable

technique(s)

IR spectroscopy: suitable

functional group

amine, carboxyl

functional group

-

functional group

-

functional group

-

cation traces

≤0.01% (Ammonia (NH3))

cation traces

Al: ≤0.0005%, As: ≤0.00001%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.001%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, NH4+: ≤0.02%, Na: ≤0.02%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

cation traces

-

cation traces

-

General description

Glycine is a non-chiral amino acid. Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking region in polyacrylamide gels. It has advantage of low mobility, hydrophobicity and it does not associate with proteins.
Glycine is a proteinogenic amino acid that plays a diverse array of roles in biochemistry and physiology. This non-essential, non-polar glucogenic amino acid acts as an inhibitory neurotransmitter in the central nervous system, but also works alongside glutamate as a co-agonist at NMDA receptors. As a precursor, glycine serves as a building block for various macromolecules, playing a crucial role in cellular processes.

Beyond its vital biological functions, glycine demonstrates remarkable versatility in various research applications. Its application as a biochemical reagent makes it invaluable in numerous assays and procedures. Its buffering capacity allows it to play a critical role in protein analysis techniques like SDS-PAGE and Western Blotting, chromatography, and cell culture. Glycine′s zwitterionic nature makes it an effective buffer across a range of pH values. In immunology research, it is widely used in the preparation of buffers for Western Blotting and other techniques. Additionally, its compatibility with various enzymes makes it useful in enzymatic assays like lactate determination. Furthermore, glycine contributes to the formulation of buffers for protein stabilization, pH control, and enzymatic reactions.

Application

  • Glycine has been added in the transfer buffer during western blotting procedure.
  • It has been used in the solution prepared for dissolving formazan crystals in the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) cytotoxicity assay.
  • It has been used for terminating the rat basophilic leukemia (RBL) assay.

Biochem/physiol Actions

Glycine is an inhibitory neurotransmitter in the central nervous system and works as an allosteric regulator of NMDA (N-methyl-D-aspartate) receptors. It is involved in processing of motor and sensory data, thereby regulating movement, vision and audition.
Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Features and Benefits

  • Suitable for Cell Biology and Biochemical research
  • High-quality compound suitable for multiple research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sigma-Aldrich

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Sigma-Aldrich

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Glycine meets analytical specification of Ph. Eur., BP, USP, 99-101% (based on anhydrous substance)

Sigma-Aldrich

15527

Glycine

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Articles

Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.

Protocols

Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.

To measure luciferase activity, an assay that measures light emission with a luminometer is used. Firefly luciferase is used for detection and quantitation of ATP and as a reporter for genetic function.

To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

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