G7141

Glucose Oxidase from Aspergillus niger

Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)

• Synonym(s): G.Od., β-D-Glucose:oxygen 1-oxidoreductase, GOx
• CAS Number: 9001-37-0
• Enzyme Commission number: 1.1.3.4 (BRENDA, IUBMB)
• EC Number: 232-601-0
• MDL number: MFCD00131182
• NACRES: NA.54

Properties

• type: Type X-S
• form: lyophilized powder
• specific activity: 100,000-250,000 units/g solid (without added oxygen)
• mol wt: 160 kDa
• composition: Protein, ≥65%
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• application(s): diagnostic assay manufacturing
• foreign activity: Catalase ≤5 units/mg protein
• greener alternative category: Enabling
• storage temp.: −20°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell research. For more information see the article in biofiles.

Molecular Weight: 160 kDa (gel filtration)
pI: 4.2
Extinction coefficient: E^1% = 16.7 (280 nm)

Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.

Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates.

The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a K_M of 33-110 mM.

Glucose oxidase does not require any activators, but it is inhibited by Ag⁺, Hg²⁺, Cu²⁺, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.

Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.

Mass of glucose oxidase (GOX), a flavoprotein ranges from approximately 130 to 175 kDa. Some fungi and insects are capable of producing GOX.

Application

Glucose Oxidase from Aspergillus niger has been used:

• in the (glucose oxidase) GO reagent to measure the glucose content by the glucose oxidase (GO) method
• to activate the human renal carcinoma cell line for constructing the oxidative stress model
• to study its influence in the paste on the analytical performance of the bioelectrode

Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors.

Packaging

10000, 50000, 250000, 1000000, 2500000 units in poly bottle

Biochem/physiol Actions

Glucose oxidase (GOX) oxidizes D-glucose to D-gluconolactone and hydrogen peroxide. Hydrogen peroxide, the catalytic product of GOX, serves as an anti-bacterial and anti-fungal agent. It is safe and can be used in several industrial applications like baking, dry egg powder production, wine production, gluconic acid production, etc. It possesses electrochemical activity, which makes it extremely important in glucose sensors and in fuel cell applications.

Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.

Quality

May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.

Unit Definition

One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H₂O₂ per min at pH 5.1 at 35 °C, equivalent to an O₂ uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.

Analysis Note

Protein determined by biuret

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves