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G7513

L-Glutamine solution

200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

L-Glutamine solution

Synonym(s):

Glavamin, Levoglutamide

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
EC Number:
200-292-1
NACRES:
NA.75
eCl@ss:
32160406
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sterility

sterile-filtered

Quality Level

product line

BioXtra

form

solution

concentration

200 mM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

rat ... Ggt1(116568)

General description

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Application

L-glutamine is an essential amino acid that is a crucial component of culture media, which serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.

Preparation Note

Prepared in cell culture grade water.

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This Item
G2150G8540G3126
form

solution

form

solution

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

product line

BioXtra

product line

-

product line

-

product line

ReagentPlus®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, residual solvents, tested

impurities

≤0.2% free ammonia., ~0.5% Glutamic acid

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200


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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protocols

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Articles

Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.

Importance and uses of glutamine in hybridoma and mammalian cell culture

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

View All Articles

Zlata Vershinin et al.
The Journal of biological chemistry, 291(13), 6786-6795 (2016-02-05)
Lysine methylation of non-histone proteins has emerged as a key regulator of many cellular functions. Although less studied than other post-translational modifications such as phosphorylation and acetylation, the number of known methylated non-histone proteins is rapidly expanding. We have identified
Miguel Pérez-Garrastachu et al.
Nucleus (Austin, Tex.), 8(5), 515-533 (2017-07-12)
Nucleoporins are the main components of the nuclear-pore complex (NPC) and were initially considered as mere structural elements embedded in the nuclear envelope, being responsible for nucleocytoplasmic transport. Nevertheless, several recent scientific reports have revealed that some nucleoporins participate in
Ofir Cohn et al.
Scientific reports, 6, 37115-37115 (2016-11-16)
SETD3 is a member of the protein lysine methyltransferase (PKMT) family, which catalyzes the addition of methyl group to lysine residues. Accumulating data suggest that PKMTs are involved in the regulation of a broad spectrum of biological processes by targeting



Global Trade Item Number

SKUGTIN
G7513-100ML04061838259547
G7513-20ML04061838081117
G7513-6X100ML04061838259554
G7513-450ML04061837035197

Questions

1–5 of 5 Questions  
  1. Guten Tag, ist das L-Glutamin biologischen Ursprungs? Danke und VG!

    1 answer
    1. This product is produced through bacterial fermentation. The process is generally considered to be of organic origin in the biochemical sense, as it is produced by living organisms (bacteria) rather than through purely chemical synthesis.

      Helpful?

  2. How much of Product G7513 should be used in cell culture media?

    1 answer
    1. In general, MEM typically contains 0.292 g/L L-Glutamine. A 200mM Glutamine solution is equivalent to 29.23 mg/mL. So it needs to be diluted around 100 times.

      Helpful?

  3. Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?

    1 answer
    1. L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled.  After thawing, it will be an opalescent solution.  If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization.  To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed.  This will expose the product to the minimal amount of warming cycles.

      Helpful?

  4. Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.

    1 answer
    1. Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

Active Filters

  1. Columbia, MO
    • Reviews 2
    • Votes 6
    5 out of 5 stars.

    L-Glutamine Soluntion

    My cell cultures have no problem growing when using just a little extra L-Glutamine.

    Helpful?

  2. 1 Ratings-only review