G9020

Gastrin I human

≥95% (HPLC), powder

• Synonym(s): Gastrin I, heptadecapeptide, HG-17, Little gastrin I, human, [18-Pyroglutamic acid]big gastrin I-(18-34)-peptide amide, human, [pGlu¹⁸]-Big gastrin I fragment 18-34 amide
• Empirical Formula (Hill Notation): C₉₇H₁₂₄N₂₀O₃₁S
• CAS Number: 10047-33-3
• Molecular Weight: 2098.20
• EC Number: 233-157-0
• MDL number: MFCD00076500
• PubChem Substance ID: 24895361
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• Assay: ≥95% (HPLC)
• form: powder
• mol wt: 2098.20
• storage condition: (Keep container tightly closed in a dry and well-ventilated place)
• concentration: ≤100%
• technique(s): protein expression: suitable
• solubility: water: 1.00-1.04 mg/mL, clear, colorless
• UniProt accession no.: P01350
• storage temp.: −20°C
• SMILES string: CSCC[C@H](NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)CNC(=O)[C@H](Cc3ccc(O)cc3)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc4c[nH]c5ccccc45)NC(=O)[C@@H]6CCCN6C(=O)CNC(=O)[C@@H]7CCC(=O)N7)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](Cc8ccccc8)C(N)=O
• InChI: 1S/C97H124N20O31S/c1-49(2)39-68(114-95(146)71(43-54-46-100-59-18-11-9-16-57(54)59)116-97(148)73-19-12-37-117(73)76(121)48-102-85(136)60-24-30-74(119)104-60)93(144)110-65(29-35-81(130)131)91(142)109-64(28-34-80(128)129)90(141)108-63(27-33-79(126)127)89(140)107-62(26-32-78(124)125)88(139)106-61(25-31-77(122)123)87(138)103-50(3)84(135)113-69(41-52-20-22-55(118)23-21-52)86(137)101-47-75(120)105-70(42-53-45-99-58-17-10-8-15-56(53)58)94(145)111-66(36-38-149-4)92(143)115-72(44-82(132)133)96(147)112-67(83(98)134)40-51-13-6-5-7-14-51/h5-11,13-18,20-23,45-46,49-50,60-73,99-100,118H,12,19,24-44,47-48H2,1-4H3,(H2,98,134)(H,101,137)(H,102,136)(H,103,138)(H,104,119)(H,105,120)(H,106,139)(H,107,140)(H,108,141)(H,109,142)(H,110,144)(H,111,145)(H,112,147)(H,113,135)(H,114,146)(H,115,143)(H,116,148)(H,122,123)(H,124,125)(H,126,127)(H,128,129)(H,130,131)(H,132,133)/t50-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,70-,71-,72-,73-/m0/s1
• InChI key: GKDWRERMBNGKCZ-RNXBIMIWSA-N
• Gene Information: human ... GAST(2520)

Description

Amino Acid Sequence

Glp-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2

General description

Research area: Cell Signaling

The GAST gene is mapped to human chromosome 17q21. Gastrin is a linear peptide. It is a gastrointestinal hormone, produced by G cells in the stomach.

Application

Gastrin I human has been used:

• to treat AGS stably transfected with gastrin receptor/cholecystokinin receptor 2 (CCK2R) (AGS-GR) cells to study the expression and secretion of clusterin (CLU) in human gastric cancer cells
• as a component of growth medium for culturing mouse normal esophageal epithelial organoid (mNEEO)
• as a supplement in basal culture media to use for organoid-like cultures
• as a molecular weight marker to calibrate superdex^® 30 columns.

Biochem/physiol Actions

Gastrin plays a major role in parietal cell function, gastric epithelial cell multiplication, and differentiation. It acts as a modulator in maintaining and organizing gastric mucosa. It is produced in the stomach and stimulates gastric acid secretion. Gastrin is an important growth factor for the gastrointestinal tract. Gastrin binds with high affinity to the cholecystokinin B receptor (CCKBR) that belongs to the family of G-protein coupled receptors. It induces islet differentiation in the pancreas.

Other Notes

Lyophilized from 0.1% TFA in H2O

Legal Information

Superdex is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves