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G9264

Sigma-Aldrich

Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse

clone GAP-7B10, ascites fluid

Synonym(s):
Anti-GAP-43
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GAP-7B10, monoclonal

mol wt

antigen 46 kDa

contains

15 mM sodium azide

species reactivity

hamster, snake, chicken, human, feline, mouse, rat

technique(s)

immunohistochemistry: suitable
western blot: 1:2,000 using newborn rat brain extract

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... GAP43(2596)
mouse ... Gap43(14432)
rat ... Gap43(29423)

General description

Monoclonal Anti-Growth Associated Protein-43 (mouse IgG2a isotype) is derived from the GAP-7B10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with HPLC-purified GAP-43 from neonatal rat forebrain membranes. Growth Associated Protein 43 (GAP-43) has a very acidic pI (4.3-4.6, depending on the species) and an anomalous behavior in SDS gels such that its reported molecular mass ranges from 43-57 kDa whereas, the primary sequence of the protein and its hydrodynamic behavior indicate a molecular mass of about 24 kDa.

Specificity

Recognizes an epitope present on both kinase C phosphorylated and dephosphorylated forms of GAP-43.

Immunogen

GAP-43 from neonatal rat forebrain membranes.

Application

Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).
Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse is suitable for:
  • immunostaining of tissue sections from the prefrontal cortex and hippocampus of rat pups to recognize an epitope present on kinase C domain in the N terminal of GAP-43 protein
  • immunohistochemistry at a working dilution of 1:3000 using sections from fetal and adult brains of mice
  • immunofluorescence at a working dilution of 1:4000 using 8μm sections of mice brain
  • western blotting using hippocampal lysates from rats
It is also suitable for immunoblotting at a working dilution of 1:2000 using newborn rat brain extract. The antibody is useful in the study of the role and function of GAP-43 during axonogenesis and in the adult nervous system.

Biochem/physiol Actions

Growth Associated Protein 43(GAP-43) precise function in growth cones and synaptic terminals is not known, increased phosphorylation of GAP-43 correlates with exocytosis, depolarization, and treatment of growth cones with NGF, implying that kinase C and possibly other protein kinase-mediated phosphorylation of GAP-43 could be a component of a common response of growth cones to diverse extracellular stimuli. GAP43 associates with the plasma membrane via acylated residues near the amino terminus and with the submembrane fraction of the cytoskeleton, presumably through its highly charged, extended carboxyl terminus, which bears similarities to neurofilament proteins. Monoclonal antibody reacting specifically against GAP-43 is a useful tool in the study of the role and function of GAP-43 during axonogenesis and in the adult nervous system.
The neural-specific Growth-Associated Protein-43 (GAP-43) is an intracellular phosphoprotein found exclusively in the peripheral and central nervous systems. The axonal growth cones that are involved in the functions of motility and pathfinding during axonogenesis and regeneration contain GAP-43. Phosphorylation of GAP-43 is associated with exocytosis, depolarization and treatment of growth cones with nerve growth factor (NGF).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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