G9269

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): GFAP Antibody Sigma - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, Gfap Antibody Sigma, Anti-GFAP
• MDL number: MFCD00145904
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rat, human
• packaging: antibody small pack of 25 μL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using brain sections; microarray: suitable; western blot: 1:500 using brain extract
• UniProt accession no.: P14136
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... GFAP(2670) ; rat ... Gfap(24387)

Description

General description

The GFAP (glial fibrillary acidic protein) gene encodes an intermediate protein filament-III (50kDa). It is localized particularly to the astrocytes and non-myelinating Schwann cells of the central and peripheral nervous system, respectively.

Specificity

The product reacts specifically with GFAP and labels astrocytes, glial cells and glial cell derived tumors in immunohistochemical staining. The antibody reacts with glial specific antigen in alcohol or formalin-fixed, paraffin-embedded human or animal tissue sections.

Immunogen

GFAP from human brain

Application

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit is suitable for:

• immunohistochemical staining at a working dilution of 1:400 using rat brain sections
• immunohistochemical staining of human brain tumor tissue specimens to identify the cells expressing AQP4 (Aquaporin-4)
• immunofluorescence using brain section from mice

The antibody is suitable for immunohistochemistry at a working dilution of 1:80 using alcohol- and formalin-fixed, paraffin-embedded sections of human or animal brain tissue. It is also suitable for indirect immunoblotting at a working dilution of 1:500 using human brain extract blot and microarray.

Biochem/physiol Actions

Glial fibrillary acidic protein may be used as a marker for distinguishing astrocytes from other glial cells during development of the central nervous system. GFAP is responsible for maintaining the structure of glial cells. It also offers mechanical strength and support to the surrounding neurons and blood brain barrier. Defects in this gene causes Alexander disease, indicated by Rosenthal fibers deposition. It is a rare disorder of astrocytes in the central nervous system. Increased GFAP expression indicates astrogliosis, a sign of neurodegeneration and neuroinflammation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at 2-8 °C up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable