H3537

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

• Synonym(s): N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• CAS Number: 7365-45-9
• MDL number: MFCD00006158
• UNSPSC Code: 12161700
• PubChem Substance ID: 329815199
• NACRES: NA.25

Properties

• Product Name: HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
• grade: BioPerformance Certified
• Quality Level: 200
• sterility: 0.2 μm filtered
• form: liquid
• concentration: 1 M
• technique(s): cell culture | mammalian: suitable
• impurities: Bioburden, tested; DNase, RNase, Protease, Nickase, free; endotoxin, tested
• pH: 5.0-6.0
• useful pH range: 6.8-8.2
• cation traces: Fe: <5 ppm; heavy metals (as Pb): <5 ppm
• suitability: suitable for molecular biology
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Application

RNAse HEPES has been used:

• To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets[3]
• To recover purified ribonucleotide[4]
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing[5]
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets[6]

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable