Merck
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H3784

Sigma-Aldrich

HEPES sodium salt

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

Assay

≥99.0%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless (260 mg/mL)

absorption

≤0.02 at 280 in H2O at 0.1 M
≤0.04 at 260 in H2O at 0.1 M

application(s)

diagnostic assay manufacturing

foreign activity

DNase, RNase, NICKase, protease, none detected

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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Sigma-Aldrich

H8651

HEPES sodium salt

grade

BioPerformance Certified

grade

-

grade

reagent grade

grade

-

assay

≥99.0%

assay

-

assay

96%

assay

≥99.0% (titration)

form

powder

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

impurities

endotoxin and total aerobic microbial count, tested

impurities

Endotoxin, microbial, and trace metals; tested

impurities

-

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

pH

10-12 (1 M in water)

pH

-

pH

-

pH

10.0-12.0 (1 M in H2O)

Physical form

Lyophilized, spray dried, or crystallized material.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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