Merck
All Photos(4)

H4034

Sigma-Aldrich

HEPES

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

Assay

≥99.5% (titration)

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

useful pH range

6.8-8.2

pKa (25 °C)

7.5

cation traces

Fe: ≤5 ppm

absorption

≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M

application(s)

diagnostic assay manufacturing

foreign activity

DNase, RNase, NICKase, protease, none detected

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
V900477PHR1428RDD002
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sigma-Aldrich

H4034

HEPES

HEPES Vetec™, reagent grade, 99.5%

Sigma-Aldrich

V900477

HEPES

HEPES Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1428

HEPES

HEPES anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD002

HEPES

grade

BioPerformance Certified

grade

reagent grade

grade

certified reference material, pharmaceutical secondary standard

grade

anhydrous

assay

≥99.5% (titration)

assay

99.5%

assay

-

assay

≥99.5%

form

crystalline powder

form

crystalline powder

form

-

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

impurities

endotoxin and total aerobic microbial count, tested

impurities

-

impurities

-

impurities

-

cation traces

Fe: ≤5 ppm

cation traces

-

cation traces

-

cation traces

-

General description

HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines
  • As a component of platelet suspension buffer
  • To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
  • As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
  • For the adjustment and maintenance of pH of biological solutions
  • As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
  • For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
  • As a component of keratinocyte and fibroblast culture medium

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service