H4034

HEPES

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• PubChem Substance ID: 24895616
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• Quality Level: 400
• Assay: ≥99.5% (titration)
• form: crystalline powder
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin and total aerobic microbial count, tested
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• cation traces: Fe: ≤5 ppm
• absorption: ≤0.05 at 260 in H₂O at 0.1 M; ≤0.05 at 280 in H₂O at 0.1 M
• application(s): diagnostic assay manufacturing
• foreign activity: DNase, RNase, NICKase, protease, none detected
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:

• To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines
• As a component of platelet suspension buffer
• To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
• As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
• For the adjustment and maintenance of pH of biological solutions
• As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
• For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
• As a component of keratinocyte and fibroblast culture medium

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves