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H4034

Sigma-Aldrich

HEPES

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

assay

≥99.5% (titration)

application(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

useful pH range

6.8 - 8.2

pKa (25 °C)

7.5

cation traces

Fe: ≤5 ppm

absorption

≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M

Featured Industry

Diagnostic Assay Manufacturing

foreign activity

DNase, RNase, NICKase, protease, none detected

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

Application

HEPES has been used:
  • To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines
  • As a component of platelet suspension buffer
  • To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
  • As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
  • For the adjustment and maintenance of pH of biological solutions
  • As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
  • For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
  • As a component of keratinocyte and fibroblast culture medium

Packaging

25 kg in poly drum
1 kg in poly bottle
100, 500 g in poly bottle

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development.
Polleux F and Ghosh A
Science's STKE : Signal Transduction Knowledge Environment, 136, pl9-pl9 (2002)
Blood compatibility of surfaces with superlow protein adsorption.
Zhang Z, et al.
Biomaterials, 29(32), 4285-4291 (2008)
Three-Dimensional Human Tissue Models of Wounded Skin
Egles C, et al.
Methods in Molecular Biology, 585, 345-359 (2010)
Complex thermorheology of living cells.
Schmidt BUS, et al.
New Journal of Physics, 17(7), 073010-073010 (2015)
Use of a new buffer in the culture of animal cells.
Williamson JD and Cox P
The Journal of General Virology, 2, 309-309 (1968)

Protocols

Protein Interactions

This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein interactions with great confidence.

cAMP Assays

cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.

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