cell culture | mammalian: suitable
endotoxin and total aerobic microbial count, tested
6.8 - 8.2
Fe: ≤5 ppm
≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M
Diagnostic Assay Manufacturing
DNase, RNase, NICKase, protease, none detected
Looking for similar products? Visit Product Comparison Guide
This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein interactions with great confidence.
cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.