All Photos(3)

H6147

Sigma-Aldrich

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

product line

BioXtra

assay

≥99.5% (titration)

form

powder

application(s)

cell culture | embryo: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8 - 8.2

pKa (25 °C)

7.5
7.5

solubility

water: 500 mg/mL, clear, colorless

absorption

≤0.05 at 290 in H2O at 33%

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Application

HEPES has been used:
  • As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
  • As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
  • in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Packaging

100, 500 g in poly bottle

Other Notes

This product has been qualified for use in cell and mouse embryo culture.
Easily compare specifications for HEPES products with the HEPES specification table.

Physical properties

Useful pH range: 6.8 - 8.2

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception.
Aslam I and Fishel S
Human Reproduction, 13(3), 634-638 (1998)
The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes.
Pancarci SM, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16, 757-763 (2010)
Viable offspring derived from cryopreserved haploid rabbit parthenotes.
Garci'a-Ximenez F and Escriba MJ
Theriogenology, 57(4), 1319-1325 (2002)
Lilianna Szyk-Warszyńska et al.
Polymers, 11(5) (2019-05-28)
Multilayer films containing α- and β-casein and polypeptides, poly-L-lysine (PLL), and poly-L-arginine (PLArg) were formed by the layer-by-layer technique and Fourier Transform InfraRed spectroscopy with Attenuated Total Reflection (FTIR-ATR) and FTIR/Grazing Angle analyzed their infrared spectra. We investigated the changes...
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)

Articles

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service