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H6903

Sigma-Aldrich

HEPBS

≥99% (titration)

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Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(4-butanesulfonic acid)
Empirical Formula (Hill Notation):
C10H22N2O4S
CAS Number:
161308-36-7
Molecular Weight:
266.36
MDL number:
MFCD00677124
NACRES:
NA.25

Quality Level

200

Assay

≥99% (titration)

form

powder

pH

7.6-9.0

useful pH range

7.6-9.0

pKa (25 °C)

8.3

pKa 

8.3

solubility

water: 0.5 g/mL, clear to slightly hazy, colorless to faintly yellow

application(s)

diagnostic assay manufacturing

InChI

1S/C10H22N2O4S/c13-9-8-12-6-4-11(5-7-12)3-1-2-10-17(14,15)16/h13H,1-10H2,(H,14,15,16)

InChI key

LOJNFONOHINEFI-UHFFFAOYSA-N

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This Item
H752354457H23830
HEPBS ≥99% (titration)

Sigma-Aldrich

H6903

HEPBS

HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

HEPES BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

54457

HEPES

HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

form

powder

form

powder

form

powder

form

crystalline

pH

7.6-9.0

pH

5.0-6.5 (1 M in H2O)

pH

5.0-6.5 (25 °C, 1 M in H2O)

pH

-

solubility

water: 0.5 g/mL, clear to slightly hazy, colorless to faintly yellow

solubility

H2O: 1 M, clear, colorless

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

useful pH range

7.6-9.0

useful pH range

6.8-8.2

useful pH range

6.8-8.2

useful pH range

6.8-8.2

General description

HEPBS/N-(2-Hydroxyethyl)piperazine-N′-(4-butanesulfonic acid) is mainly used in buffer preparations.

Linkage

Homolog of HEPES and EPPS with higher pKa and similar utility in biological systems.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315 - H319 - H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Template-Directed Synthesis in 3?-and 5?-Direction with Reversible Termination
Kaiser A, et al.
Angewandte Chemie (International Edition in English), 51(33), 8299-8303 (2012)
T Thiel et al.
Journal of biochemical and biophysical methods, 37(3), 117-129 (1998-12-31)
Four new zwitterionic butanesulfonic acid buffers that are structurally related to four families of Good buffers were evaluated for use in biological systems. These buffers, with pKa values from 7.6 to 10.7, were compared with a variety of other buffers
Michael Bachmann et al.
Journal of cell science, 133(9) (2020-03-21)
αVβ3 integrin can bind to multiple extracellular matrix proteins, including vitronectin (Vn) and fibronectin (Fn), which are often presented to cells in culture as homogenous substrates. However, in tissues, cells experience highly complex and changing environments. To better understand integrin

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