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H7541

Sigma-Aldrich

Interleukin-10 human

IL-10, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym(s):
IL-10
MDL number:
NACRES:
NA.77

Quality Level

biological source

human

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤1.5 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 17 kDa (glycosylated)

application(s)

cell culture | mammalian: suitable

impurities

≤1EU/mg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL10(3586)

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General description

Interleukin-10 gene with five exons is mapped to human chromosome 1. The gene codes for 35 kD protein containing 178 amino acids. IL-10 is a homodimer with two, non-covalently bonded monomers.

Application

Interleukin-10 human has been used to study its effect on activation of endogenous signal transducer and activator of transcription 1 (STAT1)/STAT3 in the knock-in, in A549 human lung adenocarcinoma cell line.

Packaging

10 μg in poly bottle

Biochem/physiol Actions

Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.
IL-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.

Physical form

Lyophilized from a 0.2 μm filtered solution of 10mM Tris-HCl pH 7.4 + 150mM NaCl

Preparation Note

HumanKine Interleukin-10 (IL-10) is expressed in human HEK 293 cells as a glycosylated, non-disulfide linked homodimer with an apparent molecular mass of 17 kDa due to glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse master cell line) in presence of 200 pg/mL IL-4.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc.

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Tagging of genomic STAT3 and STAT1 with fluorescent proteins and insertion of a luciferase reporter in the cyclin D1 gene provides a modified A549 cell line to screen for selective STAT3 inhibitors
Samsonov A, et al.
PLoS ONE, 8(7) (2013)
Biology of interleukin-10
Sabat R, et al.
Cytokine & Growth Factor Reviews, 21(5), 331-344 (2010)
Robert Sabat et al.
Cytokine & growth factor reviews, 21(5), 331-344 (2010-12-01)
Interleukin (IL)-10 is the most important cytokine with anti-inflammatory properties besides TGF-β and IL-35. It is produced by activated immune cells, in particular monocytes/macrophages and T cell subsets including Tr1, Treg, and Th1 cells. IL-10 acts through a transmembrane receptor...
Da-En Cheng et al.
Molecular medicine reports, 10(5), 2389-2394 (2014-09-19)
The process of differentiation from monocytes to dendritic cells is critical in immune modulation. Monocyte apoptosis is a key regulator in balancing the immune response. Galectin‑1 has been reported to induce tolerogenic dendritic cells by the autocrine interleukin (IL)‑10 in...
Piyatida Tangteerawatana et al.
The Southeast Asian journal of tropical medicine and public health, 45(3), 517-530 (2014-07-01)
Immunity to malaria can be acquired but only after repeat exposures to polymorphic Plasmodium. However, the development of clinical outcomes during P. falciparum infection is not clearly understood. This study elucidated whether monocytes, neutrophils and pro/anti-inflammatory cytokines were associated with...

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