Merck
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H9537

Sigma-Aldrich

Anti-HMG-1 antibody, Mouse monoclonal

clone HAP46.5, purified from hybridoma cell culture

Synonym(s):
Anti-Amphoterin, Anti-HMGB1, Anti-High-Mobility-Group protein
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HAP46.5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~25 kDa

species reactivity

rat, mouse, chicken, canine, human

technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extract of human MCF7 breast carcinoma cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)

General description

High-mobility group-1 protein (HMG-1) or amphoterin is a nuclear DNA-binding protein that facilitates the bending and transcription of DNA. It also has a pivotal role in the regulation of chromatin structure, DNA replication, ligation and repair. Monoclonal anti-HMG-1 antibody can be used as a vital tool to study the biology of transcription. It can also be used in indirect ELISA and immunoblotting (25kD approx.). Mouse anti-HMG-1 antibody reacts specifically with HMG-1 of canine, mouse, chicken, rat and human.
Monoclonal Anti-HMG-1 (mouse IgG1 isotype) is derived from the HAP46.5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with purified human recombinant HMG-1. HMG-1 (High-Mobility-Group protein, amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMG-1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMG-1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus.

Immunogen

purified recombinant human HMG-1.

Application

Monoclonal Anti-HMG-1 antibody is suitable for use in immunochemical applications such as ELISA and immunoblotting (approx. 25 kDa).
Monoclonal anti-HMG-1 antibody can be used in immunofluorescent staining. It can also be used in microarray and western blotting.

Biochem/physiol Actions

High-Mobility-Group protein, amphoterin, HMG-1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMG-1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice.

Suitability

Monoclonal Anti-HMG-1 recognizes human, canine, mouse, rat, and chicken HMG-1.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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