The High Mobility Group (HMG1) is secreted by activated monocytes and macrophages. The gene is mapped to human chromosome 13q12.
synthetic peptide corresponding to amino acid 165-180 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.
Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit has been used in ELISA and immunofluorescence.
In the nucleus, HMGB1 (high-mobility group protein B1) works as a nucleosome stabilizer and transcriptional regulator. It also exhibits chaperone-like activity and suppresses aggregation of polyglutamine. It has a role in inflammation and autophagy. HMGB1 can interact with toll-like receptor (TLR) 2 and 4, interleukin-1β, chemokine (C-X-C motif) ligand 12 (CXCL12) and pathogen-associated molecular pattern (PAMP) molecules. It also works as a damage-associated molecular pattern molecule (DAMP).
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.
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