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H9664

Sigma-Aldrich

Anti-HMGB1 (HMG1) (N-terminal) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa

species reactivity

human, rat, mouse

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunoprecipitation (IP): 10 μg using HEK-293T cell lysates
indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells.
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using 3T3 cell lysates

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)

General description

Anti-HMGB1 (HMG1) (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to human HMGB1 conjugated to KLH. High Mobility Group B (HMGB) protein family includes HMGB1, HMGB2 and HMGB3, that are highly conserved and indistinguishable in their biochemical properties. HMGB1 is a 25 kDa protein of 215 amino acids and consists of two homologous HMG-boxes rich in basic amino acids, and an acidic tail at the carboxy-terminus.
HMGB proteins belong to the High Mobility Group (HMG) family of proteins that contain the HMG-box for binding and changing DNA structures [1].

Immunogen

synthetic peptide corresponding to amino acids 2-17 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.

Application

Anti-HMGB1 (HMG1) (N-terminal) antibody is suitable for use in chemiluminescent immunoblot (using mouse heart homogenates) [2]. The antibody can also be used in immunoprecipitation (10 μg using HEK-293T cell lysates), indirect immunofluorescence (1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells), and western blot (1-2 μg/mL using 3T3 cell lysates).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

High Mobility Group B (HMGB-1 and 2) participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signaling.[3] It shows a defect in transcriptional enhancement of the glucocorticoid receptor, that indicates the important role for HMGB1 in proper transcriptional control by specific transcription factors. Increased expression of HMGB1 is observed in cancer cells.[4]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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