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LONG® R3 IGF-I human

recombinant, expressed in E. coli, Recombinant Analog, suitable for cell culture

Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:

Quality Level


expressed in E. coli


≥95% (HPLC)




endotoxin tested


cell culture | mammalian: suitable

storage temp.



LONG® R3 IGF-I human has been used:
  • for the in vitro direct stimulation of pancreatic islets
  • as a component of growth medium for retinal cell lines
  • to culture vascular smooth muscle cells (VSMCs)

LONG® R3 IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) that has been specifically engineered for the enhancement of cell culture performance. It is more biologically potent in vitro than either insulin or native IGF-I and has been shown to significantly increase recombinant protein production. It is ideal for both research and large-scale culture systems utilizing serum-free or low-level serum applications. LONG R3 IGF-I has been used with numerous cell types including CHO, vaccine-producing cell lines, fibroblasts and stem cells. All cell types that have a growth response to insulin in cell culture have the potential to respond to LONG R3 IGF-I. LONG R3 IGF-I provides equivalent or better performance to recombinant insulin depending on the cell line and clone.

LONG R3 IGF-I is produced in an Escherichia coli expression system without the use of animal-derived components.

Biochem/physiol Actions

A genetically engineered recombinant human analog of IGF-1.

Preparation Note

Lyophilized from 0.1M acetic acid

Legal Information

LONG is a registered trademark of Repligen Sweden AB

Storage Class Code

13 - Non Combustible Solids

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do the sequences of Products I1146 and I1271 compare to that of native Insulin-like Growth Factor 1 (IGF-1)?

    The entire IGF-1 gene codes for 194 amino acids, but the mature form of the protein is 70 amino acids, consisting of amino acids 49-118.Product I1146 is R3 IGF-1, which is also 70 amino acids in length. The Glutamic acid (E) at position 3 has simply been replaced by an Arginine (R). So the sequence of Product I1146 is:GP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAProduct I1271 is the same as I1146, but with an additional thirteen amino acids "in front of" the normal N-terminus of the R3 IGF-1:MFPAM PLSSLFVNGP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSANote that the first 13 amino acids in the recombinant I1271 (MFPAMPLSSLFVN)are quite different from what would have been the preceding 13 amino acids in the immature IGF-1.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Decreased 11beta-hydroxysteroid dehydrogenase 1 level and activity in murine pancreatic islets caused by insulin-like growth factor I overexpression
Chowdhury S, et al.
Testing, 10(8), e0136656-e0136656 (2015)
RNA interference-mediated NOTCH3 knockdown induces phenotype switching of vascular smooth muscle cells in vitro
Liu N, et al.
International Journal of Clinical and Experimental Medicine, 8, 12674-12674 (2015)
Manuel Schmidt et al.
Molecular therapy oncolytics, 16, 134-146 (2020-02-15)
Cancer cachexia is a complex metabolic disease so far lacking effective therapy, and it accounts for approximately one third of all cancer-related deaths worldwide. The extracellular ligand Wnt7a has a dual function in skeletal muscle, inducing the anabolic AKT/mammalian target...
Niels Henning Skotte et al.
Experimental neurology, 332, 113396-113396 (2020-07-06)
Huntington disease (HD) is an autosomal dominant neurodegenerative disorder caused by an expansion of a polyglutamine repeat in the huntingtin (HTT) protein. Aberrant activation of caspase-6 and cleavage of mutant HTT generating the toxic N-terminal 586 HTT fragment are important...
Richard Birk et al.
In vivo (Athens, Greece), 28(5), 795-802 (2014-09-06)
Tissue engineering represents a promising research field, targeting the creation of new functional muscle tissue in vitro. The aim of the present study was to show the influence of static magnetic fields (SMF) and insulin-like growth factor-1 (IGF1), as enhancing...

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