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Kanamycin solution from Streptomyces kanamyceticus

50 - 60 mg/mL in 0.9% NaCl, BioReagent, liquid, 0.1 μm filtered, suitable for cell culture

CAS Number:
MDL number:
PubChem Substance ID:

Quality Level

biological source

Streptomyces kanamyceticus


0.1 μm filtered

product line





50 - 60 mg/mL in 0.9% NaCl


cell culture | mammalian: suitable
cell culture | plant: suitable


endotoxin, tested

Mode of action

protein synthesis | interferes

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

storage temp.


SMILES string




InChI key


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General description

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 2 mL/L.


Kanamycin solution from Streptomyces kanamyceticus has been used:
  • to induce deafness in neonatal rats
  • as an endothelial growth medium component for culturing human umbilical vein endothelial cells (HUVECs)
  • as medium component for Mycobacteria Cultures and Top10 E coli cells

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.


Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.


Health hazard




Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

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Stem Cell Reviews and Reports, 11(5), 774-787 (2015)
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The Journal of experimental biology, 220(Pt 22), 4204-4212 (2017-09-25)
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