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K0254

Sigma-Aldrich

Kanamycin solution from Streptomyces kanamyceticus

50 - 60 mg/mL in 0.9% NaCl, BioReagent, liquid, 0.1 μm filtered, suitable for cell culture

CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.76

Quality Level

biological source

Streptomyces kanamyceticus

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

concentration

50 - 60 mg/mL in 0.9% NaCl

application(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

impurities

endotoxin, tested

Mode of action

protein synthesis | interferes

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma

storage temp.

2-8°C

SMILES string

NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H36N4O11/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17/h4-18,23-29H,1-3,19-22H2/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-/m1/s1

InChI key

SBUJHOSQTJFQJX-NOAMYHISSA-N

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General description

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 2 mL/L.

Application

Kanamycin solution from Streptomyces kanamyceticus has been used:
  • to induce deafness in neonatal rats
  • as an endothelial growth medium component for culturing human umbilical vein endothelial cells (HUVECs)
  • as medium component for Mycobacteria Cultures and Top10 E coli cells

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Caution

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 2

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

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Rosskothen-Kuhl N, et al.
Frontiers in Cellular Neuroscience, 12, 43-43 (2018)
Toll-like receptor 2 release by macrophages: an anti-inflammatory program induced by glucocorticoids and lipopolysaccharide
Hoppstadter J, et al.
Frontiers in immunology, 10, 1634-1634 (2019)
Generation of isogenic human iPS cell line precisely corrected by genome editing using the CRISPR/Cas9 system
Grobarczyk B, et al.
Stem Cell Reviews and Reports, 11(5), 774-787 (2015)
Indrikis A Krams et al.
The Journal of experimental biology, 220(Pt 22), 4204-4212 (2017-09-25)
Communities of symbiotic microorganisms that colonize the gastrointestinal tract play an important role in food digestion and protection against opportunistic microbes. Diet diversity increases the number of symbionts in the intestines, a benefit that is considered to impose no cost...
Samuel S Porter et al.
Current protocols in microbiology, 57(1), e101-e101 (2020-05-08)
This protocol describes the production of human papillomavirus (HPV)-derived quasiviruses. Quasiviruses are infectious particles that are produced in 293TT packaging cells and contain a complete viral genome. We describe methods for infection of primary human keratinocytes with HPV quasiviruses, as...

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