Merck
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L0401

Sigma-Aldrich

Lectin from Lycopersicon esculentum (tomato)

FITC conjugate, buffered aqueous solution

Synonym(s):
LEA, Lycopersicon esculentum agglutinin
NACRES:
NA.32

Quality Level

conjugate

FITC conjugate

form

buffered aqueous solution

extent of labeling

2.0-6.0 mol FITC per mol lectin

storage temp.

2-8°C

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General description

Lectins are carbohydrate binding proteins. Lectin from Lycopersicon esculentum (LEA) binds strongly to polylactosamine oligosaccharides. It is the major component of endothelial and epithelial cell surface carbohydrate.

Application

Lectin from Lycopersicon esculentum (tomato) has been used:
  • for immunofluorescence staining
  • for the quantification of vascular perfusion
  • for the evaluation of gel perfusion efficiency

Biochem/physiol Actions

Lectin has anti-cancer, anti-human immunodeficiency virus (anti-HIV), anti-microbial infection and blocks mucosal atrophy. It reduces type 2 diabetes and obesity, stimulates nutrient absorption and drug targeting. It is used to stain the vascular tree in the spinal cord and brain of animals. Lectin agglutinates cells and precipitates polysaccharides and glycoproteins.
LEA is not blood group specific, but has an affinity for N-acetyl-β-D-glucosamine oligomers. The lectin is a glycoprotein containing approx. equal amounts of protein and carbohydrate and is reported to inhibit the mitogenic activity of phytohemagglutinin from Phaseolus vulgaris. This lectin binds endothelial cells and is used to visualize the microvasculature.

Packaging

Sold on the basis of mg active conjugate.

Physical form

Solution in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca2+, 0.08% sodium azide and 5 mg/mL β-cyclodextrin.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Lectin conjugates as biospecific contrast agents for MRI. Coupling of Lycopersicon esculentum agglutinin to linear water-soluble DTPA-loaded oligomers
Pashkunova-Martic I, et al.
Molecular Imaging and Biology : MIB : The Official Publication of the Academy of Molecular Imaging, 13(3), 432-442 (2011)
Lycopersicon esculentum lectin: an effective and versatile endothelial marker of normal and tumoral blood vessels in the central nervous system
Mazzetti S, et al.
European Journal of Histochemistry, 48(4), 423-428 (2009)
Loss of serglycin promotes primary tumor growth and vessel functionality in the RIP1-Tag2 mouse model for spontaneous insulinoma formation
Hamilton A, et al.
PLoS ONE, 10(5), e0126688-e0126688 (2015)
Qiao-Sheng Wang et al.
CNS neuroscience & therapeutics (2020-06-13)
The aim of this study was to explore whether the antibrain edema of hypertonic saline (HS) is associated with alleviating ischemic blood-brain barrier (BBB) permeability by downregulating astrocyte-derived vascular endothelial growth factor (VEGF), which is mediated by microglia-derived NOD-like receptor
Shannon Hall et al.
Sleep, 43(11) (2020-06-01)
Chronic sleep restriction (CSR) negatively impacts brain functions. Whether microglia, the brain's resident immune cells, play any role is unknown. We studied microglia responses to CSR using a rat model featuring slowly rotating wheels (3 h on/1 h off), which

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