Merck
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L2654

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O26:B6

γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):
LPS
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

purified by

gel-filtration chromatography

technique(s)

cell culture | mammalian: suitable

impurities

<5% Protein (Lowry)

solubility

H2O: 5 mg/mL, slightly hazy

storage temp.

2-8°C

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Application

Lipopolysaccharides from Escherichia coli 026:B6 was used to elicit the secretion of cytokines by human PBMC, murine bone marrow dendritic cells and rat astrocytes.
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Packaging

1 mg in glass bottle

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

David Gozal et al.
Sleep, 33(3), 319-325 (2010-03-27)
Sleep disordered breathing in children is associated with severity-dependent increases in excessive daytime sleepiness (EDS). TNF-alpha is an inflammatory cytokine that has been implicated in EDS. Since, at any given level of apnea-hypopnea index, there is significant variability in EDS
Ø Salvesen et al.
Journal of neuroinflammation, 14(1), 106-106 (2017-05-24)
The cellular prion protein (PrP In order to explore putative roles for PrP All LPS-treated goats displayed clinical signs of sickness behavior, which were of significantly (p < 0.01) longer duration in animals without PrP Our data suggest that PrP
S Ménard et al.
Gut, 53(6), 821-828 (2004-05-13)
Probiotic bacteria have a beneficial effect on intestinal inflammation. In this study, we have examined the effect of lactic acid and commensal Gram positive (+) bacteria conditioned media (CM) on tumour necrosis factor alpha (TNF-alpha) release and the mechanisms involved.
Changming Lu et al.
Blood, 117(16), 4293-4303 (2011-03-01)
Dendritic cells (DCs) are potent antigen-presenting cells derived from hematopoietic progenitor cells and circulating monocytes. To investigate the role of microRNAs (miRNAs) during DC differentiation, maturation, and function, we profiled miRNA expression in human monocytes, immature DCs (imDCs), and mature
Viorel Simion et al.
JCI insight, 5(21) (2020-10-07)
Long noncoding RNAs (lncRNAs) play important roles in regulating diverse cellular processes in the vessel wall, including atherosclerosis. RNA-Seq profiling of intimal lesions revealed a lncRNA, VINAS (Vascular INflammation and Atherosclerosis lncRNA Sequence), that is enriched in the aortic intima

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