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L2762

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O26:B6

purified by gel-filtration chromatography

Synonym(s):
LPS
EC Number:
MDL number:
NACRES:
NA.25

biological source

Escherichia coli (O26:B6)

Quality Level

form

lyophilized powder

purified by

gel-filtration chromatography

impurities

<5% Protein

shipped in

ambient

storage temp.

2-8°C

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General description

This product is extracted from E. coli serotype O26:B6 and purified by gel filtration. The source strain is ATCC 12795. LPS from serotype 026:B6 is the only LPS from E. coli which exhibits short chain-length behavior on SDS-PAGE. The short chain-length of this LPS is closer to that of the mutant rough strain LPS. This LPS serotype has been used at 1 μg/ml to stimulate neutrophils.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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Lipopolysaccharides

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, exposed on the cell surface.

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