L2880

Lipopolysaccharides from Escherichia coli O55:B5

purified by phenol extraction

• Synonym(s): LPS
• EC Number: 297-473-0
• MDL number: MFCD00164401
• NACRES: NA.25

Properties

• biological source: Escherichia coli (O55:B5)
• Quality Level: 300
• form: lyophilized powder
• purified by: phenol extraction
• impurities: <3% Protein (Lowry)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Lipopolysaccharides (LPSs) consist of a lipid A moiety linked to an antigenic O-polysaccharide.

Application

Lipopolysaccharide from Escherichia coli O55:B5 has been used:

• to stimulate hepatocytes and non-parenchymal cells
• to stimulate the spleen and study the expression of the Malitaf gene in the fish blunt snout bream
• as part of the concanavalin A (ConA) and D-galactosamine/lipopolysaccharide (D-GalN/LPS) model of fulminant hepatitis to study the effect of the natural compound hesperetin

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipid A plays a role in the biological properties attributed to endotoxic LPS. LPS is a highly immunogenic antigen which has the ability to enhance immune responses.

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300
• Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves