Merck
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L3126

Sigma-Aldrich

Lipase from porcine pancreas

Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

Synonym(s):
Pancreatic lipase, Triacylglycerol lipase, PPL, Triacylglycerol acylhydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

Porcine pancreas

Quality Level

type

Type II

form

lyophilized (cake)

specific activity

≥125 units/mg protein (using olive oil (30 min incubation))
30-90 units/mg protein (using triacetin)

composition

protein, ≥20%

storage temp.

2-8°C

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Application

Lipase from porcine pancreas has been used:
  • for the analysis of fatty acid at the 2-position of triglycerides.
  • to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
  • as a component of dissolution media to study the dissolution profiles of theophylline.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Packaging

25, 100, 500 g in poly bottle

Biochem/physiol Actions

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Quality

Crude (Steapsin). Contains amylase and protease activity.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

Analysis Note

Protein determined by biuret.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

M T Andonaegui et al.
Drug development and industrial pharmacy, 25(11), 1199-1203 (1999-12-22)
Dissolution profiles of theophylline (TP) from three types of sustained-release (SR) matrix tablets (plastic [PL], lipid [LP], and hydrophilic [HP]) in different dissolution media, with and without enzymes, were established. Also investigated was the influence of a treatment of the
Peiliang Zhang et al.
Journal of pharmaceutical and biomedical analysis, 174, 486-494 (2019-06-24)
Natural compounds with diverse structural skeletons have different ionization efficiencies and different mass spectrometry (MS) responses. Some key factors influencing the electrostatic field induced spray ionization (EFISI)-MS for a variety of natural compounds have been optimized and improved. Fifteen reference
Enzymatic transesterification of rapeseed oil and lauric acid in a continuous reactor.
Forssell, P., et al.
Journal of the American Oil Chemists' Society, 70, 1105-1109 (1993)
The contribution of fruits and vegetables to dietary intake of polyphenols and antioxidant capacity in a Mexican rural diet: Importance of fruit and vegetable variety.
Hervert-Hernandez D et al.
Food Research International, 44, 1182-1189 (2011)
Wijitrapha Ruangaram et al.
Pharmaceuticals (Basel, Switzerland), 13(4) (2020-04-03)
The prevalence of obesity is increasing globally. Despite the availability of a variety of anti-obesogenic drugs, including therapies under clinical development, these treatments are often indicated for patients with severe obesity, making them unsuitable for patients with mild obesity or

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