L3771

Sigma-Aldrich

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Synonym(s):
Lauryl sulfate sodium salt, Sodium lauryl sulfate, Dodecyl sulfate sodium salt, SDS, Dodecyl sodium sulfate
Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
Molecular Weight:
288.38
Beilstein/REAXYS Number:
3599286
EC Number:
MDL number:
eCl@ss:
39093306
PubChem Substance ID:
NACRES:
NB.22

Quality Level

grade

for molecular biology

description

anionic

product line

BioReagent

assay

≥98.5% (GC)

mol wt

micellar 18,000

aggregation number

62

application(s)

electrophoresis: suitable

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

transition temp

cloud point >100 °C

solubility

water: soluble

anion traces

chloride (Cl-): ≤500 ppm
phosphate (PO43-): ≤10 ppm

cation traces

heavy metals (as Pb): ≤10 ppm

absorption

≤0.1 at 260 at 3%
≤0.1 at 280 at 3%

HLB

40

suitability

suitable for electrophoresis

foreign activity

DNase, RNase, none detected

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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General description

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Application

Sodium dodecyl sulfate has been used:
  • in chromatin immunoprecipitation
  • in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
  • as a component of radioimmunoprecipitation assay buffer for immunoblotting
Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.
Anionic detergent

Packaging

25, 100, 500 g in poly bottle
1 kg in poly bottle

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

signalword

Danger

Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

UN1325 - class 4.1 - PG 3 - Flammable solids, organic, n.o.s., HI: all

WGK Germany

WGK 2

Flash Point F

338.0 °F

Flash Point C

170 °C

Certificate of Analysis

Certificate of Origin

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