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L5293

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4

Ready Made solution, 1 mg/mL, 0.2 μm filtered

Synonym(s):
LPS
MDL number:
NACRES:
NA.25

Quality Level

biological source

Escherichia coli (O111:B4)

sterility

0.2 μm filtered

form

aqueous solution

concentration

1 mg/mL

shipped in

wet ice

storage temp.

2-8°C

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Application

Lipopolysaccharides from Escherichia coli O111:B4 has been used:
  • to stimulate an inflammatory response in porcine liver tissues in-vivo and piglet ileum tissues ex vivo
  • to stimulate Ex vivo natural killer(NK) cells and hepatic B cells
  • to induce arthritis in a standard rabbit model

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

NKG2D is critical for NK cell activation in host defense against Pseudomonas aeruginosa respiratory infection
Wesselkamper S, et al.
Journal of immunology (Baltimore, Md. : 1950), 181(8), 5481-5489 (2008)
Effects of dietary beta-glucans supplementation on cytokine expression in porcine liver
Ryan M, et al.
Journal of Animal Science, 90(suppl-4), 40-42 (2012)
Effect of maternal seaweed extract supplementation on suckling piglet growth, humoral immunity, selected microflora, and immune response after an ex vivo lipopolysaccharide challenge
Leonard SG, et al.
Journal of Animal Science, 90(2), 505-514 (2012)
Imaging of normal and pathologic joint synovium using nonlinear optical microscopy as a potential diagnostic tool
Tiwari N, et al.
Journal of Leukocyte Biology, 15(5), 056001-056001 (2010)
6 Analysis of Lipopolysaccharides of Gram-Negative Bacteria
Methods in Microbiology, 18(5), 157-207 (1985)

Related Content

Lipopolysaccharides

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, exposed on the cell surface.

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